PMID- 9389401
OWN - NLM
STAT- MEDLINE
DCOM- 19980127
LR  - 20191210
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 68
IP  - 2
DP  - 1997 Nov
TI  - Extraction and purification of active IgG from SSPE and MS brain.
PG  - 119-25
AB  - Immunoglobulin (Ig) G was purified from soluble and membrane fractions of 
      postmortem subacute sclerosing panencephalitis (SSPE) brain, multiple sclerosis 
      (MS) brain plaque-periplaque white matter, and normal human brain (NHB) white 
      matter. After homogenization in 0.32 M sucrose and removal of cell debris and 
      nuclei by low-speed centrifugation, soluble and crude membrane fractions were 
      separated by ultracentrifugation. After removal of sucrose by dialysis, IgG was 
      isolated from the soluble fraction by protein A affinity chromatography. IgG was 
      obtained from the membrane fraction by elution at low pH and purification from 
      the eluate by protein A chromatography. Whereas very little IgG was in NHB white 
      matter, significant levels of IgG were recovered from both SSPE and MS brain. 
      Both immunocytochemical staining of measles virus-infected cells in tissue 
      culture and protein immunoblotting of virus-infected cell lysates showed that the 
      IgG from SSPE brain contained activity specific for measles virus protein. The 
      abundance, purity and functional activity of IgG extracted from SSPE and MS brain 
      indicate that IgG extracted from the brain of humans with an inflammatory disease 
      of unknown etiology can be used to identify its corresponding antigen.
FAU - Owens, G P
AU  - Owens GP
AD  - Department of Neurology, University of Colorado Health Sciences Center, Denver 
      80262, USA. gregory.owens@UCHSC.edu
FAU - Burgoon, M P
AU  - Burgoon MP
FAU - Devlin, M E
AU  - Devlin ME
FAU - Gilden, D H
AU  - Gilden DH
LA  - eng
GR  - NS 07321/NS/NINDS NIH HHS/United States
GR  - NS 32308/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Nucleocapsid Proteins)
RN  - 9004-70-0 (Collodion)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*isolation & purification/metabolism
MH  - Brain Chemistry/*immunology
MH  - Cell Line
MH  - Cell Membrane/immunology
MH  - Chlorocebus aethiops
MH  - Collodion
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin G/*isolation & purification/metabolism
MH  - Isoelectric Focusing
MH  - Kidney/cytology
MH  - Measles virus/immunology
MH  - Multiple Sclerosis/*immunology
MH  - Nucleocapsid Proteins/immunology
MH  - Protein Denaturation
MH  - Solubility
MH  - Subacute Sclerosing Panencephalitis/*immunology
EDAT- 1997/12/06 00:00
MHDA- 1997/12/06 00:01
CRDT- 1997/12/06 00:00
PHST- 1997/12/06 00:00 [pubmed]
PHST- 1997/12/06 00:01 [medline]
PHST- 1997/12/06 00:00 [entrez]
AID - S0166-0934(97)00118-3 [pii]
AID - 10.1016/s0166-0934(97)00118-3 [doi]
PST - ppublish
SO  - J Virol Methods. 1997 Nov;68(2):119-25. doi: 10.1016/s0166-0934(97)00118-3.