PMID- 9389405
OWN - NLM
STAT- MEDLINE
DCOM- 19980127
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 68
IP  - 2
DP  - 1997 Nov
TI  - Differentiation of North American and European porcine reproductive and 
      respiratory syndrome virus genotypes by in situ hybridization.
PG  - 161-8
AB  - Non-radioactive probes that can detect specifically North American and European 
      isolates of porcine reproductive and respiratory syndrome virus (PRRSV) in 
      formalin-fixed paraffin-embedded tissues by in situ hybridization were developed. 
      These probes allow the differentiation between North American and European 
      genotypes of the PRRS virus as well as the detection of both genotypes. Two 
      amplified cDNA products generated by polymerase chain reaction (PCR), one from 
      the cDNA of the Canadian PRRSV LHVA-93-3 isolate and the second one from the 
      European Lelystad isolate, and labelled with digoxigenin were utilized as probes. 
      The LHVA-93-3 derived probe was found to detect Canadian and USA PRRSV isolates 
      in infected cells, while the Lelystad derived probe hybridized only with European 
      isolates. The specificity of both probes was also demonstrated on formalin-fixed 
      tissues collected from PRRSV infected pigs. Furthermore, by combining the 
      LHVA-93-3 (North American) probe and the Lelystad (European) probe, successful 
      detection of both PRRSV genotypes in fixed tissues could be achieved.
FAU - Larochelle, R
AU  - Larochelle R
AD  - Laboratoire d'hygiene veterinaire et alimentaire, Agriculture et Agroalimentaire 
      Canada, Saint-Hyacinthe, Quebec. larocheller@em.agr.ca
FAU - Magar, R
AU  - Magar R
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA Probes)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Animals
MH  - Canada
MH  - DNA Probes
MH  - Europe
MH  - Formaldehyde
MH  - Genotype
MH  - In Situ Hybridization/*methods
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*classification/*genetics/isolation & purification
MH  - Swine
MH  - Tissue Fixation
MH  - United States
EDAT- 1997/12/06 00:00
MHDA- 1997/12/06 00:01
CRDT- 1997/12/06 00:00
PHST- 1997/12/06 00:00 [pubmed]
PHST- 1997/12/06 00:01 [medline]
PHST- 1997/12/06 00:00 [entrez]
AID - S0166-0934(97)00130-4 [pii]
AID - 10.1016/s0166-0934(97)00130-4 [doi]
PST - ppublish
SO  - J Virol Methods. 1997 Nov;68(2):161-8. doi: 10.1016/s0166-0934(97)00130-4.