PMID- 9389438
OWN - NLM
STAT- MEDLINE
DCOM- 19971230
LR  - 20131121
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 29
IP  - 3
DP  - 1997 Nov 1
TI  - Acridine orange as an indicator of the cytoplasmic ribosome state.
PG  - 215-21
AB  - In hippocampal neurons of ground squirrels and neuroblastoma culture the ribosome 
      state was analyzed by staining with acridine orange (AO), labeling with 
      radioactive amino acids, and electron microscopy. Electron microscopy indicated 
      that the extent to which ribosomes associated in polysomes varied from 25% in 
      brain cells of torpid ground squirrels up to 93% in growing neuroblastoma cells. 
      In control rat neurons, it was 75%. The fluorescence of the AO-rRNA complex in 
      ribosomes changed with the polysome/monosome ratio. The red fluorescence of 
      AO-single-stranded rRNA complex as well as ribosome association in polysomes 
      decreased greatly as a function of the shift from polysomes to monosomes. The 
      green fluorescence of AO intercalated in the double-stranded rRNA changed 
      insignificantly. As a result, the ratio of red to green fluorescence intensity, 
      Kalpha, changed more than 3-fold with the changing polysome/monosome ratio. 
      Protein labeling also showed strong positive correlation with Kalpha. Thus, rRNA 
      showed different accessibility for AO binding in active and inactive ribosomes. A 
      possible mechanism of partial rRNA shielding with proteins is proposed. AO 
      fluorescence in the cytoplasm, i.e., AO binding to rRNA in ribosomes, is presumed 
      to reflect adequately the profound changes in the state of cell protein 
      synthesizing system that can be regulated by both functional activity and stress 
      factors.
FAU - Gordon, R Y
AU  - Gordon RY
AD  - Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow 
      Region. khutzian@mars.ibioc.serpukhov.su
FAU - Bocharova, L S
AU  - Bocharova LS
FAU - Kruman, I I
AU  - Kruman II
FAU - Popov, V I
AU  - Popov VI
FAU - Kazantsev, A P
AU  - Kazantsev AP
FAU - Khutzian, S S
AU  - Khutzian SS
FAU - Karnaukhov, V N
AU  - Karnaukhov VN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Ribosomal)
RN  - 98600C0908 (Cycloheximide)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Cycloheximide/pharmacology
MH  - Cytoplasm/*chemistry
MH  - *Fluorescent Dyes
MH  - Hippocampus/cytology
MH  - *Indicators and Reagents
MH  - Kinetics
MH  - Male
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Double-Stranded/analysis
MH  - RNA, Ribosomal/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Ribosomes/*chemistry
MH  - Sciuridae
MH  - Tumor Cells, Cultured
EDAT- 1997/12/06 11:03
MHDA- 2000/06/20 09:00
CRDT- 1997/12/06 11:03
PHST- 1997/12/06 11:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/06 11:03 [entrez]
AID - 10.1002/(SICI)1097-0320(19971101)29:3<215::AID-CYTO4>3.0.CO;2-A [pii]
PST - ppublish
SO  - Cytometry. 1997 Nov 1;29(3):215-21.