PMID- 9389761
OWN - NLM
STAT- MEDLINE
DCOM- 19980109
LR  - 20171116
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 45
IP  - 12
DP  - 1997 Dec
TI  - Optimization of nuclear transcript detection by FISH and combination with 
      fluorescence immunocytochemical detection of transcription factors.
PG  - 1585-92
AB  - Detection of specific nuclear transcripts by fluorescence in situ hybridization 
      (FISH) has constituted a major breakthrough in the study of the organization of 
      transcription in the cell nucleus. Using the model of heat shock genes, we 
      present an optimized procedure for nuclear transcripts that provides high 
      efficiency for RNA detection and good preservation of cell morphology and nuclear 
      texture. Using this procedure, we designed an original high-efficiency 
      methodology combining FISH and fluorescence immunocytochemistry (FICC), which is 
      used here for the simultaneous detection of heat-shock protein (hsp) nuclear 
      transcripts and the specific heat-shock transcription factor 1 (HSF1). We show 
      that the nuclear accumulation sites of HSF1 in heat-shocked cells do not 
      correspond to the sites of transcription of the hsp70 gene.
FAU - Jolly, C
AU  - Jolly C
AD  - DyOGen, INSERM U309, Institut Albert Bonniot, La Tronche, France.
FAU - Mongelard, F
AU  - Mongelard F
FAU - Robert-Nicoud, M
AU  - Robert-Nicoud M
FAU - Vourc'h, C
AU  - Vourc'h C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSF1 protein, human)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Nucleus/ultrastructure
MH  - Cells, Cultured
MH  - DNA/analysis
MH  - DNA-Binding Proteins/*analysis/genetics
MH  - *Fluorescent Antibody Technique, Indirect
MH  - HSP70 Heat-Shock Proteins/*analysis/genetics
MH  - Heat Shock Transcription Factors
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - RNA/analysis
MH  - Transcription Factors/*analysis
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - 10.1177/002215549704501201 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1997 Dec;45(12):1585-92. doi: 10.1177/002215549704501201.