PMID- 9390716
OWN - NLM
STAT- MEDLINE
DCOM- 19980206
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 700
IP  - 1-2
DP  - 1997 Oct 24
TI  - Simultaneous determination of amphetamine and its analogs in human whole blood by 
      gas chromatography-mass spectrometry.
PG  - 77-82
AB  - A sensitive and specific gas chromatography-mass spectrometry (GC-MS) method for 
      the determination of amphetamine (AM), methamphetamine (MA), 
      methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA) and 
      methylenedioxyethylamphetamine (MDEA) in whole blood was designed, using the 
      respective pentadeuterated analogs of the analytes as internal standards (I.S.). 
      After alkalinisation of blood samples, the amphetamines were extracted using 
      diethyl ether, derivatized with heptafluorobutyric anhydride, then purified by 
      successive washings with deionized water and 4% NH4OH. Extraction recoveries were 
      85.2% for AM, 90.9% for MA, 76.5% for MDA, 84.1% for MDMA and 63.6% for MDEA. 
      Chromatographic separation was performed on a non-polar 30 m x 0.32 mm HP 5 MS 
      capillary column using a temperature program. Detection was carried out in the 
      electron-impact, selected ion-monitoring mode, using three mass-to-charge ratios 
      for each analyte and one for each I.S. Limits of detection ranged from 0.5 to 8 
      ng/ml and limits of quantification were 10 ng/ml for AM, MDMA and MDEA; 20 ng/ml 
      for MA; and 50 ng/ml for MDA. The method was linear from this limit up to 1000 
      ng/ml for all analytes, with good intra-assay precision and good intermediate 
      precision and accuracy over these ranges. There was no interferences from other 
      sympathomimetic drugs such as ephedrine, norephedrine or methoxyphenamine. This 
      method is thus suitable for clinical and forensic toxicology, as well as for 
      doping control.
FAU - Marquet, P
AU  - Marquet P
AD  - Department of Pharmacology and Toxicology, University Hospital, Limoges, France.
FAU - Lacassie, E
AU  - Lacassie E
FAU - Battu, C
AU  - Battu C
FAU - Faubert, H
AU  - Faubert H
FAU - Lachatre, G
AU  - Lachatre G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Stimulants)
SB  - IM
MH  - Amphetamines/*blood
MH  - Central Nervous System Stimulants/*blood
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 1997/12/09 00:00
MHDA- 1997/12/09 00:01
CRDT- 1997/12/09 00:00
PHST- 1997/12/09 00:00 [pubmed]
PHST- 1997/12/09 00:01 [medline]
PHST- 1997/12/09 00:00 [entrez]
AID - 10.1016/s0378-4347(97)00318-6 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1997 Oct 24;700(1-2):77-82. doi: 
      10.1016/s0378-4347(97)00318-6.