PMID- 9393765
OWN - NLM
STAT- MEDLINE
DCOM- 19980302
LR  - 20091119
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 57
IP  - 23
DP  - 1997 Dec 1
TI  - Met and hepatocyte growth factor/scatter factor expression in human gliomas.
PG  - 5391-8
AB  - Using double immunofluorescence staining and quantitative confocal laser scan 
      microscopy, we show that the intensity of hepatocyte growth factor/scatter factor 
      (HGF/SF) and Met staining in human primary brain tumors increases with the grade 
      of malignancy and is prevalent in both the infiltrating tumor cells and 
      endothelial hyperplastic areas. HGF/SF and Met also are expressed in vitro in 
      glioblastoma multiforme cell lines as well as in normal human astrocyte (NHA) 
      cells. Moreover, HGF/SF stimulates tyrosine phosphorylation of Met in both glioma 
      cell lines and NHA cells, but only the glioma cell lines proliferate and become 
      motile and invasive in response to HGF/SF, whereas the NHA cells are 
      nonresponsive. These results implicate autocrine/paracrine Met-HGF/SF signaling 
      in glioma tumorigenesis and suggest that HGF/SF signaling through Met is 
      negatively regulated in NHA cells.
FAU - Koochekpour, S
AU  - Koochekpour S
AD  - ABL-Basic Research Program, National Cancer Institute, Frederick Cancer Research 
      and Development Center, Maryland 21702, USA.
FAU - Jeffers, M
AU  - Jeffers M
FAU - Rulong, S
AU  - Rulong S
FAU - Taylor, G
AU  - Taylor G
FAU - Klineberg, E
AU  - Klineberg E
FAU - Hudson, E A
AU  - Hudson EA
FAU - Resau, J H
AU  - Resau JH
FAU - Vande Woude, G F
AU  - Vande Woude GF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Recombinant Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Astrocytes/cytology/*metabolism
MH  - Brain Neoplasms/*metabolism/pathology
MH  - Cell Division/drug effects
MH  - Glioblastoma/metabolism/pathology
MH  - Glioma/*metabolism/pathology
MH  - Hepatocyte Growth Factor/*biosynthesis/pharmacology
MH  - Humans
MH  - Mice
MH  - Morphogenesis
MH  - Neoplasm Invasiveness
MH  - Proto-Oncogene Proteins c-met/*biosynthesis
MH  - Recombinant Proteins/biosynthesis
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1997/12/11 00:00
MHDA- 1997/12/11 00:01
CRDT- 1997/12/11 00:00
PHST- 1997/12/11 00:00 [pubmed]
PHST- 1997/12/11 00:01 [medline]
PHST- 1997/12/11 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1997 Dec 1;57(23):5391-8.