PMID- 9395100
OWN - NLM
STAT- MEDLINE
DCOM- 19980113
LR  - 20191102
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 105
IP  - 5 Pt 1
DP  - 1997 Oct
TI  - Expression of T-cell receptor Vbeta2, 6 and 8 gene families in chronic adult 
      periodontal disease.
PG  - 397-404
AB  - Substantial evidence exists to suggest a role for T-cells in periodontal disease. 
      As yet, however, the T-cell receptors remain to be characterised at the molecular 
      level. The expression and the nucleotide sequence of genes from the T-cell 
      receptor beta variable (TCRBV) gene families 2, 6 and 8 were analyzed in 
      periodontal tissue from 24 patients with chronic adult periodontal disease (CAPD) 
      and peripheral blood lymphocytes (PBL) of 16 of these patients. A restriction in 
      the expression of these TCRBV gene families was detected in periodontal tissue 
      from 14/24 patients with CAPD, and the pattern of gene expression was often 
      different between individual patients; however there was no restriction in TCRBV 
      gene expression in matched PBL samples from 8 of these 14 patients. Quantitative 
      RT PCR analysis of samples from 5 CAPD patients who expressed all 3 TCRBV gene 
      families in their periodontal tissues did not reveal any significant differences 
      in the levels of gene expression in periodontal tissue and PBL. In contrast to 
      the findings with some CAPD patients, genes from all 3 TCRBV families were always 
      expressed in periodontal tissue and PBL from disease-free control subjects. PCR 
      products from both the PBL and periodontal tissue of CAPD patients were cloned 
      and sequenced; analysis of the nucleotide sequence revealed diversity with 
      respect to the expression of TCRB joining (TCRBJ) and TCRB diversity (TCRBD) 
      genes and the sequence of the junctional region in all samples analysed. In 
      conclusion, in CAPD, the pattern of TCRBV gene expression in periodontal tissue 
      is often but not always different from that in PBL and healthy periodontal 
      tissue, which may indicate, in some cases, a local influence on particular T-cell 
      subsets which is relevant to the pathogenesis of periodontal disease. However, 
      the expressed TCRB genes are heterogeneous at the nucleotide level, emphasising 
      the underlying complexity at the molecular level in the local T-cell response in 
      CAPD.
FAU - Geatch, D R
AU  - Geatch DR
AD  - Department of Oral Biology, The Dental School, University of Newcastle upon Tyne, 
      UK.
FAU - Ross, D A
AU  - Ross DA
FAU - Heasman, P A
AU  - Heasman PA
FAU - Taylor, J J
AU  - Taylor JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (T cell receptor Vbeta8)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Chronic Disease
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Gene Rearrangement, beta-Chain T-Cell Antigen Receptor/genetics
MH  - Genes, T-Cell Receptor beta/*genetics
MH  - Genetic Variation
MH  - Humans
MH  - Lymphocytes/metabolism
MH  - Molecular Sequence Data
MH  - Periodontal Diseases/blood/*immunology/pathology
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes/immunology/metabolism
MH  - Transcription, Genetic
EDAT- 1997/12/12 00:00
MHDA- 1997/12/12 00:01
CRDT- 1997/12/12 00:00
PHST- 1997/12/12 00:00 [pubmed]
PHST- 1997/12/12 00:01 [medline]
PHST- 1997/12/12 00:00 [entrez]
AID - 10.1111/j.1600-0722.1997.tb02136.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 1997 Oct;105(5 Pt 1):397-404. doi: 
      10.1111/j.1600-0722.1997.tb02136.x.