PMID- 9397161
OWN - NLM
STAT- MEDLINE
DCOM- 19971230
LR  - 20081121
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 174
IP  - 1
DP  - 1998 Jan
TI  - Induction of hepatocyte growth factor/scatter factor by interferon-gamma in human 
      leukemia cells.
PG  - 107-14
AB  - Induction of hepatocyte growth factor/scatter factor (HGF/SF) may be one of the 
      critical steps in organ regeneration, wound healing, and embryogenesis. We 
      previously reported the production of HGF/SF from various human leukemia cell 
      lines and a high level of the growth factor in blood and bone marrow plasma from 
      patients with various types of leukemia. We determined here the effects of 
      hematopoietic cytokines on HGF/SF production in human leukemia cell lines, KG-1, 
      a myeloid cell line, and RPMI-8226, a B cell line. Interferon (IFN)-gamma 
      remarkably stimulated HGF/SF production in both cell lines at concentrations of 
      more than 0.1 or 1 IU/ml. IFN-alpha and IFN-beta were as effective as IFN-gamma 
      in RPMI-8226 cells, but less than IFN-gamma in KG-1 cells. HGF/SF gene expression 
      in KG-1 cells was also up-regulated by IFN-gamma. Granulocyte colony-stimulating 
      factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), 
      interleukin (IL)-5 and IL-6 had no effect on HGF/SF production in the 2 leukemia 
      cell lines. We also determined the effects of HGF/SF inducers known for human 
      fibroblasts on the growth factor production in leukemia cells. Out of phorbol 
      12-myristate 13-acetate (PMA), cholera toxin, IL-1 beta, and tumor necrosis 
      factor (TNF)-alpha, the former three were as effective as IFN-gamma in KG-1 
      cells, but only TNF-alpha stimulated HGF/SF production in RPMI-8226 cells, whose 
      effect was less than those of IFN-alpha, IFN-beta, and IFN-gamma. The effect of 
      IFN-gamma in KG-1 cells was synergistic with that of PMA. In contrast with the 
      effect in leukemia cells, HGF/SF induction by IFN-gamma in human skin fibroblasts 
      was much less than that by PMA or cholera toxin. These results indicated that 
      IFN-gamma is a potent inducer of HGF/SF in human leukemia cells. This finding 
      suggests the presence of a homeostatic control mechanism in liver regeneration 
      and repair: hepatic injury, DNA synthesis inhibition, or apoptosis caused by 
      IFN-gamma is subsequently overcome by cytokine-induced HGF/SF, a potent promoter 
      of liver DNA synthesis.
FAU - Gohda, E
AU  - Gohda E
AD  - Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama 
      University, Japan. gohda@pheasant.pharm.okayama-u.ac.jp
FAU - Takebe, T
AU  - Takebe T
FAU - Sotani, T
AU  - Sotani T
FAU - Nakamura, S
AU  - Nakamura S
FAU - Minowada, J
AU  - Minowada J
FAU - Yamamoto, I
AU  - Yamamoto I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Hepatocyte Growth Factor/*biosynthesis/genetics
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Leukemia/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 1997/12/16 02:44
MHDA- 2000/06/20 09:00
CRDT- 1997/12/16 02:44
PHST- 1997/12/16 02:44 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/16 02:44 [entrez]
AID - 10.1002/(SICI)1097-4652(199801)174:1<107::AID-JCP12>3.0.CO;2-C [pii]
AID - 10.1002/(SICI)1097-4652(199801)174:1<107::AID-JCP12>3.0.CO;2-C [doi]
PST - ppublish
SO  - J Cell Physiol. 1998 Jan;174(1):107-14. doi: 
      10.1002/(SICI)1097-4652(199801)174:1<107::AID-JCP12>3.0.CO;2-C.