PMID- 9397972
OWN - NLM
STAT- MEDLINE
DCOM- 19980114
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 101
IP  - 1-2
DP  - 1994 May
TI  - Cytokines modulate type I iodothyronine deiodinase mRNA levels and enzyme 
      activity in FRTL-5 rat thyroid cells.
PG  - R31-5
AB  - Tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and 
      interferon-gamma (INF-gamma) have inhibitory effects on thyroid function both in 
      vivo and in vitro. We have studied the effects of these cytokines on type I 
      5'-deiodinase (5'-DI) mRNA expression and enzyme activity in FRTL-5 cells 
      maintained in standard cell culture medium containing 0 (5H) or 2 mIU/ml bovine 
      TSH (6H). Northern blots were hybridized with 5'-DI cDNA. 5'-DI mRNA levels were 
      reduced to 20% of control values after treating cells with 100 ng/ml TNF-alpha in 
      6H for 2 days while the corresponding enzyme activity was reduced 50%. Other 
      cytokines, including IL-1beta and interferon-gamma, also significantly inhibited 
      expression of 5'-DI in FRTL-5 cells grown in 6H medium. Because the majority of 
      circulating T3 in the rat is secreted by the thyroid gland, the highly 
      significant decline in the serum T3/T4 ratio following in vivo administration of 
      cytokines may be due to their direct inhibitory effect on thyroidal 5'-DI 
      expression.
FAU - Pekary, A E
AU  - Pekary AE
AD  - Endocrinology Research Laboratory, West Los Angeles VA Medical Center, University 
      of California, 90073, USA.
FAU - Berg, L
AU  - Berg L
FAU - Santini, F
AU  - Santini F
FAU - Chopra, I
AU  - Chopra I
FAU - Hershman, J M
AU  - Hershman JM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - EC 1.11.1.8 (Iodide Peroxidase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Cytokines/*pharmacology
MH  - Iodide Peroxidase/*biosynthesis
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Thyroid Gland/*metabolism
EDAT- 1994/05/01 00:00
MHDA- 1997/12/16 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1997/12/16 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 0303-7207(94)90256-9 [pii]
AID - 10.1016/0303-7207(94)90256-9 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1994 May;101(1-2):R31-5. doi: 10.1016/0303-7207(94)90256-9.