PMID- 9398668
OWN - NLM
STAT- MEDLINE
DCOM- 20000728
LR  - 20240322
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 8
IP  - 12
DP  - 1997 Dec
TI  - Interaction of class I human leukocyte antigen (HLA-I) molecules with insulin 
      receptors and its effect on the insulin-signaling cascade.
PG  - 2463-74
AB  - Insulin receptor (IR) and class I major histocompatibility complex molecules 
      associate with one another in cell membranes, but the functional consequences of 
      this association are not defined. We found that IR and human class I molecules 
      (HLA-I) associate in liposome membranes and that the affinity of IR for insulin 
      and its tyrosine kinase activity increase as the HLA:IR ratio increases over the 
      range 1:1 to 20:1. The same relationship between HLA:IR and IR function was found 
      in a series of B-LCL cell lines. The association of HLA-I and IR depends upon the 
      presence of free HLA heavy chains. All of the effects noted were reduced or 
      abrogated if liposomes or cells were incubated with excess HLA-I light chain, 
      beta2-microglobulin. Increasing HLA:IR also enhanced phosphorylation of insulin 
      receptor substrate-1 and the activation of phosphoinositide 3-kinase. HLA-I 
      molecules themselves were phosphorylated on tyrosine and associated with 
      phosphoinositide 3-kinase when B-LCL were stimulated with insulin.
FAU - Ramalingam, T S
AU  - Ramalingam TS
AD  - Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, 
      USA.
FAU - Chakrabarti, A
AU  - Chakrabarti A
FAU - Edidin, M
AU  - Edidin M
LA  - eng
GR  - R01 AI014584/AI/NIAID NIH HHS/United States
GR  - R37-AI14584/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Glycophorins)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (IRS1 protein, human)
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Liposomes)
RN  - 0 (Phosphoproteins)
RN  - 0 (beta 2-Microglobulin)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - B-Lymphocytes/cytology/drug effects/enzymology/metabolism
MH  - Cell Line
MH  - Energy Transfer
MH  - Enzyme Activation/drug effects
MH  - Fluorescence
MH  - Fluorescent Dyes
MH  - Glycophorins/metabolism
MH  - HLA-A2 Antigen/chemistry/*metabolism/*pharmacology
MH  - Humans
MH  - Insulin/metabolism/*pharmacology
MH  - Insulin Receptor Substrate Proteins
MH  - Kinetics
MH  - Liposomes/drug effects/metabolism
MH  - Phenotype
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation/drug effects
MH  - Phosphotyrosine/metabolism
MH  - Protein Binding
MH  - Receptor, Insulin/*metabolism
MH  - Signal Transduction/*drug effects
MH  - Thermodynamics
MH  - beta 2-Microglobulin/metabolism/pharmacology
PMC - PMC25720
EDAT- 1997/12/17 02:42
MHDA- 2000/08/06 11:00
CRDT- 1997/12/17 02:42
PHST- 1997/12/17 02:42 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 1997/12/17 02:42 [entrez]
AID - 10.1091/mbc.8.12.2463 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1997 Dec;8(12):2463-74. doi: 10.1091/mbc.8.12.2463.