PMID- 9398863
OWN - NLM
STAT- MEDLINE
DCOM- 19971223
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 99
IP  - 2
DP  - 1997 Dec
TI  - Interphase detection of BCL6/IgH fusion gene in non-Hodgkin lymphoma by 
      fluorescence in situ hybridization.
PG  - 102-7
AB  - We characterized a t(3;14)(q27;q32) translocation in nine patients with B-cell, 
      non-Hodgkin lymphoma (B-NHL) by fluorescence in situ hybridization (FISH). 
      Fluorescence in situ hybridization with immunoglobulin heavy chain (IgH) and BCL6 
      gene probes detected t(3;14) rapidly and accurately, including complex t(3;14) in 
      three patients; one with t(3;12;8;14)(q27;p13;q24.1;q32) and two with 
      t(3;?;14)(q27;?;q32). Among these nine patients, seven escaped from cytogenetic 
      detection by our G-banding analysis. Double-color FISH with IgH (Y6) and BCL6 
      (cosB5-1) showed fusion of BCL6 and IgH genes on der(3)t(3;14) in all nine 
      patients, suggesting that der(3) may play a critical role in the development of 
      lymphoma carrying complex as well as standard t(3;14) translocations. BCL6/IgH 
      fusion gene was also demonstrated in interphase nuclei at a frequency of 23% to 
      91.5% over the cut-off value in control studies (9.0 +/- 2.76%). The breakpoints 
      assessed by FISH with two cosmid clones containing BCL6 probes, cosB5-1 and 
      cosB5-2, were within the cluster region in seven patients including one with 
      complex type, but were not evaluated in two patients with t(3;?;14), because of 
      the loss of partner chromosome. Using double-color FISH with these two 
      BCL6-specific probes, none of an additional 32 patients in whom mitotic spreads 
      were available showed 3q27 translocations. Fluorescence in situ hybridization 
      with IgH and BCL6 gene probes is a rapid and sensitive method to detect t(3;14) 
      in routine cytogenetic studies.
FAU - Ueda, Y
AU  - Ueda Y
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Nishida, K
AU  - Nishida K
FAU - Miki, T
AU  - Miki T
FAU - Horiike, S
AU  - Horiike S
FAU - Kaneko, H
AU  - Kaneko H
FAU - Yokota, S
AU  - Yokota S
FAU - Misawa, S
AU  - Misawa S
FAU - Abe, T
AU  - Abe T
FAU - Kashima, K
AU  - Kashima K
FAU - Taniwaki, M
AU  - Taniwaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aged
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Lymphoma, Non-Hodgkin/*genetics
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Transcription Factors/*genetics
MH  - *Translocation, Genetic
EDAT- 1997/12/17 00:00
MHDA- 1997/12/17 00:01
CRDT- 1997/12/17 00:00
PHST- 1997/12/17 00:00 [pubmed]
PHST- 1997/12/17 00:01 [medline]
PHST- 1997/12/17 00:00 [entrez]
AID - S0165460897002033 [pii]
AID - 10.1016/s0165-4608(97)00203-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Dec;99(2):102-7. doi: 10.1016/s0165-4608(97)00203-3.