PMID- 9398864
OWN - NLM
STAT- MEDLINE
DCOM- 19971223
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 99
IP  - 2
DP  - 1997 Dec
TI  - Specific cytogenetic aberrations in two novel human prostatic cell lines 
      immortalized by human papillomavirus type 18 DNA.
PG  - 108-15
AB  - Using chromosome banding and fluorescence in situ hybridization (FISH) with 
      painting probes, sequential cytogenetic analysis was performed of two novel 
      prostate cell lines, PZ-HPV-7 and CA-HPV-10, established by human papillomavirus 
      (HPV) 18 DNA transformation. PZ-HPV-7 originates from a normal diploid prostate 
      epithelial cell strain. PZ-HPV-7 progressed from an initial diploid to a 
      hypertetraploid chromosome number with a relative gain of chromosomes 5 and 20 (7 
      to 8 copies each). Structural changes were limited; 3p- (2 copies), 3q- (1 copy), 
      and possibly a der(16p;12q). CA-HPV-10 originates from an epithelial cell strain 
      derived from a high-grade human prostate cancer specimen, which showed several 
      karyotypic abnormalities including an extra Y chromosome and double minutes 
      (dmin). In early passage the karyotype of CA-HPV-10 appeared unstable with a 
      decreasing number of cells exhibiting dmin. In late passage the dmin were 
      replaced by a large homogeneously staining region (hsr) on 9p+ marker. The hsr 
      was shown by FISH to be of chromosome 1 origin. The modal number was mainly 
      hypertriploid (72, range 69 to 75). Loss of Y was remarkable (0 to 1 copy). 
      Consistent markers included two copies each of del(1)(q12q31) and 
      der(9)t(1;9)(?;p22), and one der(11)t(4;11) (?;q21). HPV type 18 genomic 
      integration sites were identified on 1p for PZ-HPV-7 and on the 9p+ marker for 
      CA-HPV-10. In conclusion, both PZ-HPV-7 and CA-HPV-10 showed clonal cytogenetic 
      changes. These two cell lines constitute a novel in vitro model to study the 
      mechanisms involved in human prostate carcino-genesis.
FAU - Weijerman, P C
AU  - Weijerman PC
AD  - Department of Urology, Erasmus University Rotterdam, The Netherlands.
FAU - van Drunen, E
AU  - van Drunen E
FAU - Konig, J J
AU  - Konig JJ
FAU - Teubel, W
AU  - Teubel W
FAU - Romijn, J C
AU  - Romijn JC
FAU - Schroder, F H
AU  - Schroder FH
FAU - Hagemeijer, A
AU  - Hagemeijer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Viral)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Cell Line, Transformed
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - *DNA, Viral
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Papillomaviridae/*genetics
MH  - Ploidies
MH  - Prostatic Neoplasms/*genetics
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1997/12/17 00:00
MHDA- 1997/12/17 00:01
CRDT- 1997/12/17 00:00
PHST- 1997/12/17 00:00 [pubmed]
PHST- 1997/12/17 00:01 [medline]
PHST- 1997/12/17 00:00 [entrez]
AID - S0165460897002070 [pii]
AID - 10.1016/s0165-4608(97)00207-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Dec;99(2):108-15. doi: 
      10.1016/s0165-4608(97)00207-0.