PMID- 9399355
OWN - NLM
STAT- MEDLINE
DCOM- 19980129
LR  - 20190905
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 17
IP  - 11
DP  - 1997 Nov
TI  - A magnetic colloid system for isolation of rare cells from blood for fish 
      analysis.
PG  - 1059-66
AB  - To improve cell recovery of trophoblast and nucleated red blood cells from 
      maternal blood for diagnosis of chromosomal abnormalities, we have investigated 
      the use of a magnetic sorting system that utilizes a ferrofluid. The main 
      advantage of this system is that the beads used are small enough to allow 
      visualization of chromosome-specific sequences by fluorescence in situ 
      hybridization (FISH). The ferrofluid was validated using MAb340, a 
      trophoblast-specific antibody, and anti-CD71, used to sort for nucleated red 
      blood cells. Antigen-positive cells could be efficiently sorted from a 1000-fold 
      excess of antigen-negative cells and easily stained by FISH. We are currently 
      evaluating its use on maternal blood samples.
FAU - Martin, B
AU  - Martin B
AD  - Department of Obstetrics and Gynaecology, City Hospital, Nottingham, U.K.
FAU - Bruce, J
AU  - Bruce J
FAU - Smith, N
AU  - Smith N
FAU - Liu, D
AU  - Liu D
FAU - Durrant, L
AU  - Durrant L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Colloids)
SB  - IM
MH  - *Blood Cells
MH  - Cell Count
MH  - Cell Separation
MH  - *Colloids
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Magnetics
MH  - Male
MH  - Microspheres
MH  - Tumor Cells, Cultured
EDAT- 1997/12/17 02:44
MHDA- 2000/06/20 09:00
CRDT- 1997/12/17 02:44
PHST- 1997/12/17 02:44 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/17 02:44 [entrez]
AID - 10.1002/(SICI)1097-0223(199711)17:11<1059::AID-PD196>3.0.CO;2-9 [pii]
AID - 10.1002/(sici)1097-0223(199711)17:11<1059::aid-pd196>3.0.co;2-9 [doi]
PST - ppublish
SO  - Prenat Diagn. 1997 Nov;17(11):1059-66. doi: 
      10.1002/(sici)1097-0223(199711)17:11<1059::aid-pd196>3.0.co;2-9.