PMID- 9399634
OWN - NLM
STAT- MEDLINE
DCOM- 19971229
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 61
IP  - 24
DP  - 1997
TI  - The transcription of the XRCC1 gene in spleen following ionizing irradiation in 
      radiosensitive and radioresistant mice.
PG  - 2417-23
AB  - The XRCC1 gene was described to play a role for the sensitivity of mammalian cell 
      lines towards ionizing irradiation. Cells with a mutation of this gene present 
      with decreased single strand break repair, reduced recombination repair, show 
      increased double strand breaks and sister chromatid exchange is increased up to 
      tenfold. The goal of our study was to investigate the transcription of this gene 
      in the spleen following ionizing irradiation in the mouse. Furthermore, we 
      intended to examine whether radiation sensitive (RS) mice would show a 
      transcriptional pattern different from radiation resistant (RR) mice. Radiation 
      sensitive BALB/c/J Him mice and radiation resistant C3H He/Him mice were 
      untreated or whole body irradiated with X-ray at 4 and 6 Gy and sacrificed 5, 15 
      and 30 min after irradiation. mRNA was isolated from the spleen and hybridized 
      with probes for XRCC1 and beta-actin as a house keeping gene control. 
      Transcription of XRCC1 was not different in unirradiated or 4 Gy-irradiated mouse 
      RR or RS mouse strains. When irradiated at 6 Gy, RR mice showed an approximately 
      threefold increase of mRNA XRCC1/mRNA beta actin as early as 15 min after 
      irradiation. We conclude that radiation resistant mice show a higher 
      transcription level for the XRCC1 gene in the spleen early after high dose X-ray 
      whole body irradiation. This finding is the first in vivo study on XRCC1 of this 
      kind and may in part explain the differences in the radiation sensitivity between 
      the two strains studied.
FAU - Labudova, O
AU  - Labudova O
AD  - University of Vienna, Department of Pediatrics, Austria.
FAU - Hardmeier, R
AU  - Hardmeier R
FAU - Golej, J
AU  - Golej J
FAU - Rink, H
AU  - Rink H
FAU - Lubec, G
AU  - Lubec G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (X-ray Repair Cross Complementing Protein 1)
RN  - 0 (Xrcc1 protein, mouse)
SB  - IM
MH  - Animals
MH  - *DNA Repair
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - *Radiation Tolerance
MH  - Spleen/metabolism/*radiation effects
MH  - *Transcription, Genetic
MH  - X-ray Repair Cross Complementing Protein 1
EDAT- 1997/01/01 00:00
MHDA- 1997/12/17 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/12/17 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S0024320597009582 [pii]
AID - 10.1016/s0024-3205(97)00958-2 [doi]
PST - ppublish
SO  - Life Sci. 1997;61(24):2417-23. doi: 10.1016/s0024-3205(97)00958-2.