PMID- 9400488
OWN - NLM
STAT- MEDLINE
DCOM- 19971229
LR  - 20190621
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 159
IP  - 1
DP  - 1998 Jan
TI  - Fluorescence in situ hybridization on nuclei from paraffin-embedded tissue in low 
      stage pure embryonal carcinoma of the testis.
PG  - 240-4
AB  - Approximately 30% of patients who present with clinical stage A nonseminomatous 
      testis cancer are in fact pathologic stage B. In previous studies an increasing 
      volume of embryonal carcinoma in the orchiectomy specimen was associated with a 
      higher likelihood of being pathologic stage B. However, not all patients with 
      pure embryonal carcinoma in the primary tumor were pathologic stage B. In an 
      effort to discriminate patients with pure embryonal carcinoma in the testicular 
      specimen relative to pathologic stage, archival specimens from patients 
      presenting with clinical stage A pure embryonal carcinoma were examined by 
      fluorescence in situ hybridization (FISH) with newly developed probes for 
      chromosome arms 12p and 12q. Whole nuclei from archival material from 14 patients 
      (six pathologic stage A, seven pathologic stage B and one stage C) with 100% 
      embryonal carcinoma in the orchiectomy specimen were studied using bicolor FISH 
      with chromosome arm 12p- and 12q-specific painting probes developed by chromosome 
      microdissection. In all cases a blinded analysis showed distinct regions of 12p 
      and 12q probe hybridization simultaneously and allowed identification of probable 
      normal chromosomes 12, as well as regions of amplification of 12p sequences, 
      including possible i(12p). In 5/14 specimens, a distinct and peculiar pattern of 
      12p hybridization was observed which resembled 12p "disarray" or "multifocal 
      12p". Of the five specimens demonstrating multifocal 12p, four were pathologic 
      stage B, while one was pathologic stage A. Whether the trend toward multifocal 
      12p predicts metastatic potential in primary testicular embryonal carcinoma will 
      need to be assessed using a larger series of patients.
FAU - Blough, R I
AU  - Blough RI
AD  - Department of Medical and Molecular Genetics, Indiana University Medical Center, 
      Indianapolis 46202-5251, USA.
FAU - Heerema, N A
AU  - Heerema NA
FAU - Albers, P
AU  - Albers P
FAU - Foster, R S
AU  - Foster RS
LA  - eng
GR  - R35 CA39844/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
SB  - IM
MH  - Carcinoma, Embryonal/*genetics/pathology
MH  - *Cell Nucleus
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm Staging
MH  - Testicular Neoplasms/*genetics/pathology
EDAT- 1997/12/24 00:00
MHDA- 1997/12/24 00:01
CRDT- 1997/12/24 00:00
PHST- 1997/12/24 00:00 [pubmed]
PHST- 1997/12/24 00:01 [medline]
PHST- 1997/12/24 00:00 [entrez]
AID - S0022-5347(01)64075-8 [pii]
AID - 10.1016/s0022-5347(01)64075-8 [doi]
PST - ppublish
SO  - J Urol. 1998 Jan;159(1):240-4. doi: 10.1016/s0022-5347(01)64075-8.