PMID- 9400834
OWN - NLM
STAT- MEDLINE
DCOM- 19971230
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 62
IP  - 6
DP  - 1997 Dec
TI  - Monocyte chemoattractant protein-2 is a potent agonist of CCR2B.
PG  - 911-5
AB  - The binding and functional activity of the CC chemokines monocyte chemoattractant 
      protein-1 (MCP-1), MCP-2, and MCP-3 have been characterized using Chinese hamster 
      ovary DXB-11 cells transfected with the chemokine receptor CCR2B. Receptor 
      binding studies demonstrated that 125I-labeled MCP-1 bound to a single class of 
      high-affinity receptors with a Kd of 0.14 (0.07-0.32) nM. In competition studies 
      MCP-1, MCP-2, and MCP-3 completely inhibited 125I-labeled MCP-1 binding with Ki 
      values of 0.3 (0.16-0.46), 8.8 (3.4-26), and 12.2 (0.6-22) nM, respectively. In 
      calcium mobilization studies, MCP-1 and MCP-3 induced robust elevations in 
      intracellular calcium concentrations, whereas MCP-2 was only weakly active. In 
      contrast, using changes in extracellular acidification rate as a functional 
      readout, all three chemokines were identified as potent agonists of CCR2B. These 
      data demonstrate that MCP-2, in addition to MCP-1 and MCP-3, is a potent agonist 
      of CCR2B and furthermore that MCP-2 activates either different or a subset of the 
      signaling pathways activated by MCP-1 and MCP-3.
FAU - Moore, U M
AU  - Moore UM
AD  - Rhone-Poulenc Rorer Ltd., Dagenham Research Centre, Essex, United Kingdom. 
      Una.Moore@DRC.RP-Rorer.rp.fr
FAU - Kaplow, J M
AU  - Kaplow JM
FAU - Pleass, R D
AU  - Pleass RD
FAU - Castro, S W
AU  - Castro SW
FAU - Naik, K
AU  - Naik K
FAU - Lynch, C N
AU  - Lynch CN
FAU - Daly, S
AU  - Daly S
FAU - Roach, A G
AU  - Roach AG
FAU - Jaye, M
AU  - Jaye M
FAU - Williams, R J
AU  - Williams RJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CCL8 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Cytokine)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Chemokine CCL8
MH  - Cricetinae
MH  - Humans
MH  - Monocyte Chemoattractant Proteins/metabolism/*pharmacology
MH  - Receptors, CCR2
MH  - *Receptors, Chemokine
MH  - Receptors, Cytokine/*agonists/genetics/metabolism
MH  - *Signal Transduction
MH  - Transfection
EDAT- 1997/12/24 00:00
MHDA- 1997/12/24 00:01
CRDT- 1997/12/24 00:00
PHST- 1997/12/24 00:00 [pubmed]
PHST- 1997/12/24 00:01 [medline]
PHST- 1997/12/24 00:00 [entrez]
AID - 10.1002/jlb.62.6.911 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1997 Dec;62(6):911-5. doi: 10.1002/jlb.62.6.911.