PMID- 9401104
OWN - NLM
STAT- MEDLINE
DCOM- 19980114
LR  - 20041117
IS  - 0003-3995 (Print)
IS  - 0003-3995 (Linking)
VI  - 40
IP  - 3
DP  - 1997
TI  - Clinical applications of primed in situ labelling (PRINS) rapid identification of 
      a marker chromosome in a fetus.
PG  - 154-7
AB  - Marker chromosomes pose a serious problem in clinical cytogenetic diagnosis since 
      the conventional banding analyses are often not useful in identifying their 
      origin or composition. In the absence of information, counseling as to the 
      clinical significance and prognosis is difficult, especially in prenatal 
      diagnosis. With the introduction of fluorescence in situ hybridization (FISH) 
      marker identification has became feasible. However, FISH is relatively 
      time-consuming and expensive. In an effort to overcome these disadvantages, we 
      have used primed in situ labelling (PRINS) technique as an alternative. Presented 
      here is one case in which PRINS was useful in rapidly identifying the origin of a 
      marker chromosome detected on amniotic fluid chromosome analysis. Based on our 
      experience with this case and others, we propose that PRINS can become a viable 
      and cost effective alternative to FISH and is as reliable as FISH in terms of 
      accuracy, specificity, and sensitivity.
FAU - Velagaleti, G V
AU  - Velagaleti GV
AD  - Clinical and Molecular Cytogenetics Laboratory, The University of Tennessee, 
      Memphis, Tennessee, USA.
FAU - Carpenter, N J
AU  - Carpenter NJ
FAU - Tharapel, A T
AU  - Tharapel AT
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - Netherlands
TA  - Ann Genet
JT  - Annales de genetique
JID - 0370562
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Amniocentesis
MH  - Chromosome Mapping
MH  - Female
MH  - Fetus/*physiology
MH  - *Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - *Polymerase Chain Reaction
MH  - Pregnancy
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 1997/01/01 00:00
MHDA- 1997/12/24 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/12/24 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Ann Genet. 1997;40(3):154-7.