PMID- 9402513
OWN - NLM
STAT- MEDLINE
DCOM- 19980306
LR  - 20191211
IS  - 1053-2498 (Print)
IS  - 1053-2498 (Linking)
VI  - 16
IP  - 11
DP  - 1997 Nov
TI  - Increased concentration of soluble human leukocyte antigen class I levels in the 
      bronchoalveolar lavage of human pulmonary allografts.
PG  - 1135-40
AB  - BACKGROUND: Human leukocyte antigen (HLA) class I antigens, are glycoproteins 
      expressed on the cell surface and are also secreted (sHLA) into the surrounding 
      fluids. This study investigates whether pulmonary allograft rejection is 
      associated with an increased amount of sHLA class I in the bronchoalveolar lavage 
      (BAL) fluid. METHODS: Enzyme-linked immunosorbant assays were used to measure 
      sHLA class I levels in BAL samples from 66 lung transplant recipients. RESULTS: 
      Analysis of pulmonary allograft recipients revealed that the mean concentration 
      of sHLA class I was significantly higher in samples from recipients with acute 
      rejection than in recipients with no evidence of rejection. Seventy-two percent 
      of the patients with rejection had sHLA levels above the normal range for 
      patients with infection or rejection. Conversely, sHLA levels were within normal 
      limits in 94% of the control population. Time kinetic analysis revealed that in 
      subjects with rejection, sHLA class I levels peaked in the first 2 weeks after 
      transplantation and decreased thereafter. Increased levels of sHLA were found in 
      patients with acute rejection but not in those with chronic rejection. Fifty-nine 
      percent (n = 10) of the patients with infection had sHLA levels within the normal 
      (no infection or rejection) range. The remaining 41% (n = 7) with infection had 
      sHLA above the normal range. Fifty-seven percent (n = 4) of the latter group had 
      cytomegalovirus infection. These results were confirmed in a prospective study 
      carried out by analyzing paired samples obtained during and after rejection. 
      Eight of the nine patients had high BAL sHLA class I during acute rejection, and 
      low BAL sHLA class I levels after resolution of the acute rejection. CONCLUSION: 
      Our data demonstrate that measurement of sHLA class I levels in BAL samples is a 
      sensitive indicator of acute rejection.
FAU - Rizzo, M
AU  - Rizzo M
AD  - Department of Surgery, Washington University School of Medicine, St. Louis, Mo. 
      63110-1093, USA.
FAU - Sundaresan, S
AU  - Sundaresan S
FAU - Lynch, J
AU  - Lynch J
FAU - Trulock, E P
AU  - Trulock EP
FAU - Cooper, J
AU  - Cooper J
FAU - Patterson, G A
AU  - Patterson GA
FAU - Mohanakumar, T
AU  - Mohanakumar T
LA  - eng
GR  - HL56643/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Heart Lung Transplant
JT  - The Journal of heart and lung transplantation : the official publication of the 
      International Society for Heart Transplantation
JID - 9102703
RN  - 0 (HLA Antigens)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Bronchoalveolar Lavage Fluid/*immunology
MH  - Chronic Disease
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Graft Rejection/immunology
MH  - HLA Antigens/*analysis
MH  - Humans
MH  - Infections/immunology
MH  - *Lung Transplantation
MH  - Membrane Glycoproteins/analysis
MH  - Middle Aged
MH  - Postoperative Complications/immunology
MH  - Prospective Studies
MH  - Time Factors
MH  - Transplantation, Homologous
EDAT- 1997/12/24 00:00
MHDA- 1997/12/24 00:01
CRDT- 1997/12/24 00:00
PHST- 1997/12/24 00:00 [pubmed]
PHST- 1997/12/24 00:01 [medline]
PHST- 1997/12/24 00:00 [entrez]
PST - ppublish
SO  - J Heart Lung Transplant. 1997 Nov;16(11):1135-40.