PMID- 9402659
OWN - NLM
STAT- MEDLINE
DCOM- 19980217
LR  - 20071114
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 15
IP  - 6
DP  - 1997
TI  - Requirement of major histocompatibility complex-compatible microenvironment for 
      spleen colony formation (CFU-S on day 12 but not on day 8).
PG  - 461-8
AB  - To clarify major histocompatibility complex (MHC) restriction between 
      hematopoietic stem cells (HSCs) and microenvironments, T cell-depleted bone 
      marrow cells (BMCs) were transplanted into MHC-compatible and MHC-incompatible 
      recipients. A significantly larger number of spleen colony-forming units (CFU-S) 
      on day 12 were noted in MHC-compatible recipients, while only a small number were 
      observed in MHC-incompatible recipients. There was, however, no significant 
      difference in CFU-S counts on day 8 between the two groups. A large number of 
      CFU-S counts on day 12 were also observed in F1 hybrid recipients, as seen in 
      syngeneic recipients. The decrease in CFU-S counts on day 12 in MHC-incompatible 
      recipients was also observed even after in vivo abrogation of T and NK cells. The 
      difference in CFU-S counts on day 12 became more prominent when HSC-enriched 
      cells were transferred. These results suggest that an MHC restriction exists 
      between pluripotent HSCs (P-HSCs) and spleen microenvironments. Furthermore, 
      experiments using B10. A recombinant strains revealed that H-2D and S loci play a 
      crucial role in the MHC restriction. The experiments of serial transplantation 
      suggest that the differentiation and proliferation of P-HSCs are inhibited in 
      MHC-incompatible microenvironments. It is therefore likely that the 
      MHC-compatible microenvironment is essential to the differentiation and 
      proliferation of P-HSCs.
FAU - Sugiura, K
AU  - Sugiura K
AD  - First Department of Pathology, Kansai Medical University, Osaka, Japan.
FAU - Inaba, M
AU  - Inaba M
FAU - Hisha, H
AU  - Hisha H
FAU - Borisov, K
AU  - Borisov K
FAU - Sardina, E E
AU  - Sardina EE
FAU - Good, R A
AU  - Good RA
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
GR  - AGO5627/AG/NIA NIH HHS/United States
GR  - AI22360/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Animals
MH  - Colony-Forming Units Assay
MH  - *Hematopoiesis
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*cytology
MH  - Histocompatibility Testing
MH  - *Major Histocompatibility Complex
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Spleen/*cytology
MH  - Stromal Cells/cytology
MH  - Time Factors
EDAT- 1997/01/01 00:00
MHDA- 1997/12/24 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/12/24 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/stem.150461 [doi]
PST - ppublish
SO  - Stem Cells. 1997;15(6):461-8. doi: 10.1002/stem.150461.