PMID- 9404984 OWN - NLM STAT- MEDLINE DCOM- 19980107 LR - 20190914 IS - 0273-8481 (Print) IS - 0273-8481 (Linking) VI - 18 IP - 6 DP - 1997 Nov-Dec TI - Thermal injury functionally alters bone marrow-derived macrophages: a study of monocyte-hepatocyte interactions. PG - 505-14 AB - Thermal injury quantitatively and qualitatively alters hematopoiesis, including monocyte-macrophage lineage changes, resulting in altered mononuclear cell function. These bone marrow cells (BMCs) ultimately become fixed tissue macrophages (e.g., Kupffer cells). To study the effects of thermal injury on macrophage-hepatocyte interactions, rat BMCs were isolated 24 hours after burn injury, and myelopoiesis was induced by 7-day culture in granulocyte-macrophage colony-stimulating factor. Separate cultures included inflammatory mediators with growth factor function (IL-6 or PGE2). Cultured cells were incubated up to 96 hours with isolated normal hepatocytes (+/- lipopolysaccharide stimulation). The 96-hour exposure to postburn BMCs produced less of the acute phase proteins (APPs), C3 and transferrin, but more cytotoxicity as measured by 1-lactate dehydrogenase release. Sham BMCs cultured with added IL-6 caused higher APP release and minimal cytotoxicity, whereas burn BMCs stimulated lower APP release and retained cytotoxicity. In conclusion, myeloid cells regulate APP synthesis differently after thermal injury and may become more cytotoxic to hepatocytes. FAU - Sternfeld, D C AU - Sternfeld DC AD - Shriners Burns Institute, and the Department of Surgery, University of Cincinnati, Ohio 45229-3095, USA. FAU - Ogle, C K AU - Ogle CK FAU - Valente, J F AU - Valente JF FAU - Noel, J G AU - Noel JG FAU - Warden, G D AU - Warden GD LA - eng PT - Journal Article PL - United States TA - J Burn Care Rehabil JT - The Journal of burn care & rehabilitation JID - 8110188 RN - 0 (Acute-Phase Proteins) RN - 0 (Transferrin) RN - EC 3.4.21.- (Complement C3-C5 Convertases) SB - IM MH - Acute-Phase Proteins/biosynthesis MH - Animals MH - Bone Marrow Cells/*physiology MH - Burns/*immunology/physiopathology MH - Cell Communication MH - Cell Differentiation MH - Complement C3-C5 Convertases/biosynthesis MH - Cytotoxicity, Immunologic/*physiology MH - Hematopoiesis/*physiology MH - In Vitro Techniques MH - Kupffer Cells/*physiology MH - Liver/cytology/physiopathology MH - Male MH - Rats MH - Rats, Inbred ACI MH - Transferrin/biosynthesis EDAT- 1997/12/24 00:00 MHDA- 1997/12/24 00:01 CRDT- 1997/12/24 00:00 PHST- 1997/12/24 00:00 [pubmed] PHST- 1997/12/24 00:01 [medline] PHST- 1997/12/24 00:00 [entrez] AID - 10.1097/00004630-199711000-00007 [doi] PST - ppublish SO - J Burn Care Rehabil. 1997 Nov-Dec;18(6):505-14. doi: 10.1097/00004630-199711000-00007.