PMID- 9405407
OWN - NLM
STAT- MEDLINE
DCOM- 19980122
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 272
IP  - 51
DP  - 1997 Dec 19
TI  - Activation of stress-activated protein kinase/c-Jun N-terminal kinase, but not 
      NF-kappaB, by the tumor necrosis factor (TNF) receptor 1 through a TNF 
      receptor-associated factor 2- and germinal center kinase related-dependent 
      pathway.
PG  - 32102-7
AB  - A key step by which tumor necrosis factor (TNF) signals the activation of nuclear 
      factor-kappaB (NF-kappaB) and the stress-activated protein kinase (SAPK, also 
      called c-Jun N-terminal kinase or JNK) is the recruitment to the TNF receptor of 
      TNF receptor-associated factor 2 (TRAF2). However, the subsequent steps in 
      TRAF2-induced SAPK and NF-kappaB activation remain unresolved. Here we report the 
      identification of a TNF-responsive serine/threonine protein kinase termed GCK 
      related (GCKR) that likely signals via mitogen-activated protein kinase 
      (MAPK)/extracellular signal-regulated kinase (ERK) kinase kinase 1 (MEKK1) to 
      activate the SAPK pathway. TNF, TRAF2, and ultraviolet (UV) light, which in part 
      uses the TNF receptor signaling pathway, all increased GCKR activity. A TRAF2 
      mutant, which inhibits both TRAF2-induced NF-kappaB and SAPK activation, blocked 
      TNF-induced GCKR activation. Finally, interference with GCKR expression impeded 
      TRAF2- and TNF-induced SAPK activation but not that of NF-kappaB. This suggests a 
      divergence in the TNF signaling pathway that leads to SAPK and NF-kappaB 
      activation, which is located downstream of TRAF2 but upstream of GCKR.
FAU - Shi, C S
AU  - Shi CS
AD  - B Cell Molecular Immunology Section, Laboratory of Immunoregulation, NIAID, 
      National Institutes of Health, Bethesda, Maryland 20892-1876, USA.
FAU - Kehrl, J H
AU  - Kehrl JH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (Germinal Center Kinases)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (TNF Receptor-Associated Factor 1)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cell Line
MH  - Enzyme Activation
MH  - Enzyme Induction
MH  - Germinal Center Kinases
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - *Mitogen-Activated Protein Kinases
MH  - Molecular Sequence Data
MH  - NF-kappa B/*metabolism
MH  - Protein Serine-Threonine Kinases/biosynthesis/chemistry/*metabolism
MH  - Proteins/*metabolism
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Sequence Homology, Amino Acid
MH  - TNF Receptor-Associated Factor 1
EDAT- 1998/01/24 00:00
MHDA- 1998/01/24 00:01
CRDT- 1998/01/24 00:00
PHST- 1998/01/24 00:00 [pubmed]
PHST- 1998/01/24 00:01 [medline]
PHST- 1998/01/24 00:00 [entrez]
AID - S0021-9258(18)39735-7 [pii]
AID - 10.1074/jbc.272.51.32102 [doi]
PST - ppublish
SO  - J Biol Chem. 1997 Dec 19;272(51):32102-7. doi: 10.1074/jbc.272.51.32102.