PMID- 9406536
OWN - NLM
STAT- MEDLINE
DCOM- 19980203
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 69
IP  - 23
DP  - 1997 Dec 1
TI  - DNA typing of human leukocyte antigen sequence polymorphisms by peptide nucleic 
      acid probes and MALDI-TOF mass spectrometry.
PG  - 4894-8
AB  - A novel analytical method using PNA probes detected by matrix-assisted laser 
      desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) was applied 
      to type sequence polymorphisms within the human leukocyte antigen (HLA), DQA 
      locus. Streptavidin-coated magnetic beads were used to immobilize biotinylated 
      DNA. PNA probes representing possible alleles were then prepared for the 
      immobilized DNA hybridization. The nonspecific PNA probes were removed with 
      stringent washes. The PNA/DNA/beads conjugate was analyzed by MALDI-TOFMS. The 
      genotype of the DNA was determined by the detected molecular masses of the 
      released PNA probes. Reproducible and accurate genotyping was achieved by this 
      analytical method.
FAU - Jiang-Baucom, P
AU  - Jiang-Baucom P
AD  - Department of Chemistry, American University, Washington, D.C. 20016, USA.
FAU - Girard, J E
AU  - Girard JE
FAU - Butler, J
AU  - Butler J
FAU - Belgrader, P
AU  - Belgrader P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (HLA Antigens)
RN  - 0 (Oligonucleotide Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*chemistry
MH  - HLA Antigens/*chemistry
MH  - Humans
MH  - Oligonucleotide Probes/*chemistry
MH  - Polymorphism, Genetic
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 1997/12/24 00:00
MHDA- 1997/12/24 00:01
CRDT- 1997/12/24 00:00
PHST- 1997/12/24 00:00 [pubmed]
PHST- 1997/12/24 00:01 [medline]
PHST- 1997/12/24 00:00 [entrez]
AID - 10.1021/ac970639u [doi]
PST - ppublish
SO  - Anal Chem. 1997 Dec 1;69(23):4894-8. doi: 10.1021/ac970639u.