PMID- 9407716
OWN - NLM
STAT- MEDLINE
DCOM- 19980127
LR  - 20061115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 82
IP  - 5
DP  - 1997 Sep-Oct
TI  - Response of myelodysplastic syndrome bone marrow cells to multiple cytokine 
      stimulation in liquid cultures: an in situ hybridization study.
PG  - 532-6
AB  - BACKGROUND AND OBJECTIVE: Myelodysplastic syndrome progenitor cells can be grown 
      and expanded in long term bone marrow liquid cultures in the presence of multiple 
      cytokines. In this study we investigated the pattern of differentiation and 
      response to growth factors in six cases of myelodysplastic syndrome (MDS) with 
      well-defined cytogenetic abnormalities by means of conventional cytogenetics and 
      fluorescence in situ hybridization (FISH). METHODS: Bone marrow cells were grown 
      in stroma-free liquid cultures in the presence of SCF, IL-3, IL-6 and GM-CSF. 
      RESULTS: IN three cases a CFU-GM expansion comparable to normal controls was 
      observed, together with a decrease or increase of cells with abnormal karyotype. 
      Two cases showed no response to growth factor stimulation, morphological signs of 
      terminal myeloid differentiation and increase (one case) or decrease (one case) 
      in the percentage of abnormal FISH signals along the cultures. In one additional 
      case, while CFU-C expansion was present, clearcut leukemic transformation was 
      observed in the culture, together with a sharp decrease in the percentage of 
      abnormal FISH signals, indicating a leukemic transformation of MDS progenitor 
      cells with a normal karyotype. INTERPRETATION AND CONCLUSIONS: Our data indicate 
      that FISH analysis is generally a poor indicator of clonality in MDS; 
      nevertheless, determining the kinetics of cytogenetically abnormal clones in 
      liquid bone marrow cultures may provide insight as to the growth abnormalities of 
      MDS progenitor cells and may be useful prior to in vivo growth factor 
      administration.
FAU - Bossolasco, P
AU  - Bossolasco P
AD  - Fondazione Matarelli, Centro Trapianti di Midollo Ospedale Maggiore IRCCS, Milan, 
      Italy.
FAU - Soligo, D
AU  - Soligo D
FAU - Servida, F
AU  - Servida F
FAU - Romitti, L
AU  - Romitti L
FAU - Caneva, L
AU  - Caneva L
FAU - Deliliers, G L
AU  - Deliliers GL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - 0 (Cytokines)
SB  - IM
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Cells, Cultured
MH  - Cytokines/*pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Myelodysplastic Syndromes/genetics/*metabolism/pathology
EDAT- 1998/01/04 00:00
MHDA- 1998/01/04 00:01
CRDT- 1998/01/04 00:00
PHST- 1998/01/04 00:00 [pubmed]
PHST- 1998/01/04 00:01 [medline]
PHST- 1998/01/04 00:00 [entrez]
PST - ppublish
SO  - Haematologica. 1997 Sep-Oct;82(5):532-6.