PMID- 9408746
OWN - NLM
STAT- MEDLINE
DCOM- 19980113
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 20
IP  - 4
DP  - 1997 Dec
TI  - Analyses of brain tumor cell lines confirm a simple model of relationships among 
      fluorescence in situ hybridization, DNA index, and comparative genomic 
      hybridization.
PG  - 311-9
AB  - Several techniques are commonly used for genetic analysis of interphase nuclei. 
      Flow cytometry assays the distribution of DNA content in populations of nuclei 
      stained with a DNA-specific fluorochrome. Fluorescence in situ hybridization 
      (FISH) quantifies the number of copies of a specific DNA sequence in single 
      nuclei. Comparative genomic hybridization (CGH) assesses the relative copy number 
      of DNA sequences throughout a test genome by comparing the signal intensities of 
      test and reference DNA samples hybridized to a template of normal metaphase 
      chromosomes. In principle, there are specific relationship among data obtained 
      from these measurements, and combined measurements should provide a more 
      comprehensive view of the sample that is analyzed. We applied these three 
      techniques to nine brain tumor cell lines and find that a model of CGH that 
      includes unsuppressed repeat sequences describes the data well. We estimate that 
      up to 35% of the fluorescence intensity in well-blocked CGH preparations may not 
      represent unique sequences. Taking these factors into account, our results are, 
      in general, mutually consistent, and highlight issues critical for interpreting 
      CGH preparations.
FAU - Mohapatra, G
AU  - Mohapatra G
AD  - Cancer Genetics Program, UCSF Cancer Center 94143-0808, USA.
FAU - Moore, D H
AU  - Moore DH
FAU - Kim, D H
AU  - Kim DH
FAU - Grewal, L
AU  - Grewal L
FAU - Hyun, W C
AU  - Hyun WC
FAU - Waldman, F M
AU  - Waldman FM
FAU - Pinkel, D
AU  - Pinkel D
FAU - Feuerstein, B G
AU  - Feuerstein BG
LA  - eng
GR  - CA13525/CA/NCI NIH HHS/United States
GR  - CA61147/CA/NCI NIH HHS/United States
GR  - CA64898/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Algorithms
MH  - Animals
MH  - Brain Neoplasms/*genetics
MH  - DNA Probes
MH  - DNA, Neoplasm/*analysis
MH  - Flow Cytometry
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Models, Genetic
MH  - Nucleic Acid Hybridization/*methods
MH  - Tumor Cells, Cultured
EDAT- 1997/12/31 23:47
MHDA- 2000/06/20 09:00
CRDT- 1997/12/31 23:47
PHST- 1997/12/31 23:47 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/31 23:47 [entrez]
AID - 10.1002/(SICI)1098-2264(199712)20:4<311::AID-GCC1>3.0.CO;2-4 [pii]
AID - 10.1002/(sici)1098-2264(199712)20:4<311::aid-gcc1>3.0.co;2-4 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Dec;20(4):311-9. doi: 
      10.1002/(sici)1098-2264(199712)20:4<311::aid-gcc1>3.0.co;2-4.