PMID- 9408748
OWN - NLM
STAT- MEDLINE
DCOM- 19980113
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 20
IP  - 4
DP  - 1997 Dec
TI  - Human/mouse microcell hybrid based elimination test reduces the putative tumor 
      suppressor region at 3p21.3 to 1.6 cM.
PG  - 329-36
AB  - We have previously identified an approximately 7 cM long common eliminated region 
      (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI-7947, 
      D3S2354, AFMb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma 
      microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of 
      our more detailed analysis on 24 SCID mouse tumors derived from two MCH lines 
      that originally carried intact human chromosomes 3. They were analyzed by 
      fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers 
      covering the region between D3S1611 and D3S13235 at 3p22-p21.2. D3S32 and D3S2354 
      were regularly eliminated during in vivo tumor growth, whereas the other 22 
      markers, D3S1611, ACAA, D3S1260, WI-692, AP20R, D3S3521, D3S966, D3S1029, D3S643, 
      WI-2420, MSTI. GNAI2, D3S1235, D3S1298, GLBI, WI-4193, D3S3658, D3S3559, D3S3678, 
      WI-6400, WI-7947, and WI-10865, were regularly retained. We have defined a common 
      eliminated region of approximately 1.6 cM (designated as CER1) inside the 7 cM 
      CER described earlier. CER1 is flanked distally by D3S1029 and proximally by 
      D3S643.
FAU - Szeles, A
AU  - Szeles A
AD  - Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. 
      anna.szeles@mtc.ki.se
FAU - Yang, Y
AU  - Yang Y
FAU - Sandlund, A M
AU  - Sandlund AM
FAU - Kholodnyuk, I
AU  - Kholodnyuk I
FAU - Kiss, H
AU  - Kiss H
FAU - Kost-Alimova, M
AU  - Kost-Alimova M
FAU - Zabarovsky, E R
AU  - Zabarovsky ER
FAU - Stanbridge, E
AU  - Stanbridge E
FAU - Klein, G
AU  - Klein G
FAU - Imreh, S
AU  - Imreh S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (CER1 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - *Chromosome Deletion
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Cytokines
MH  - DNA, Neoplasm/analysis
MH  - Fibroblasts/cytology
MH  - Fibrosarcoma/pathology
MH  - *Genes, Tumor Suppressor
MH  - Genetic Markers
MH  - Humans
MH  - Hybrid Cells/*cytology
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, SCID
MH  - Microsatellite Repeats
MH  - Polymerase Chain Reaction
EDAT- 1997/12/31 23:47
MHDA- 2000/06/20 09:00
CRDT- 1997/12/31 23:47
PHST- 1997/12/31 23:47 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/31 23:47 [entrez]
AID - 10.1002/(SICI)1098-2264(199712)20:4<329::AID-GCC3>3.0.CO;2-3 [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Dec;20(4):329-36.