PMID- 9408749
OWN - NLM
STAT- MEDLINE
DCOM- 19980113
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 20
IP  - 4
DP  - 1997 Dec
TI  - Characterization of a hairy cell leukemia-associated 5q13.3 inversion breakpoint.
PG  - 337-46
AB  - Previous cytogenetic analysis has indicated that chromosome anomalies involving 
      the 5q13 band are common in hairy cell leukemia (HCL), occurring in approximately 
      1/3 of the patients. The data suggest that 5q13.3 is likely to harbor a gene 
      involved in the transformational event of this disease. We selected a 
      constitutional inv(5)(p13.1q13.3) in a patient with HCL as the starting point in 
      an attempt to identify the relevant gene in 5q13.3. By using double color 
      interphase fluorescence in situ hybridization (FISH) techniques, we have 
      identified two cosmid probes from a chromosome 5-specific library that flank the 
      5q13.3 inversion breakpoint proximally and distally. Pulsed field gel 
      electrophoresis (PFGE) and interphase FISH experiments suggest that the two 
      markers are at a distance of no more than 300 kb. YAC probes covering a 21 Mb 
      region at 5q13 were used to map the 5q13.3 inversion breakpoint and the 
      breakpoint is located within the D5S646-D5S620 region. Two non-chimeric YACs have 
      been identified that span the breakpoint. FISH analysis revealed that four other 
      patients with cytogenetic aberrations of 5q carried inversions/deletions that 
      involved the same 5q13.3 breakpoint region. The identification of a gene involved 
      in hairy cell leukemogenesis in this region will be of major importance in the 
      elucidation of the transformational events of HCL.
FAU - Wu, X
AU  - Wu X
AD  - Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
FAU - Merup, M
AU  - Merup M
FAU - Juliusson, G
AU  - Juliusson G
FAU - Jansson, M
AU  - Jansson M
FAU - Stellan, B
AU  - Stellan B
FAU - Grander, D
AU  - Grander D
FAU - Zabarovsky, E
AU  - Zabarovsky E
FAU - Liu, Y
AU  - Liu Y
FAU - Spasokoukotskaja, T
AU  - Spasokoukotskaja T
FAU - Gahrton, G
AU  - Gahrton G
FAU - Einhorn, S
AU  - Einhorn S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Deletion
MH  - *Chromosome Inversion
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 5/*genetics
MH  - Cosmids
MH  - DNA Probes
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Genes, Neoplasm
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Leukemia, Hairy Cell/*genetics
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Tumor Cells, Cultured
EDAT- 1997/12/31 23:47
MHDA- 2000/06/20 09:00
CRDT- 1997/12/31 23:47
PHST- 1997/12/31 23:47 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/31 23:47 [entrez]
AID - 10.1002/(SICI)1098-2264(199712)20:4<337::AID-GCC4>3.0.CO;2-2 [pii]
AID - 10.1002/(sici)1098-2264(199712)20:4<337::aid-gcc4>3.0.co;2-2 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Dec;20(4):337-46. doi: 
      10.1002/(sici)1098-2264(199712)20:4<337::aid-gcc4>3.0.co;2-2.