PMID- 9408758
OWN - NLM
STAT- MEDLINE
DCOM- 19980113
LR  - 20231213
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 20
IP  - 4
DP  - 1997 Dec
TI  - Characterization of a t(10;12)(q24;p13) in a case of CML in transformation.
PG  - 408-11
AB  - We have used Southern blotting and fluorescence in situ hybridization (FISH) to 
      define the breakpoints of a reciprocal translocation, t(10;12)(q24;p13), acquired 
      as a secondary abnormality in a patient with Philadelphia chromosome positive 
      chronic myeloid leukemia (CML) in transformation. A YAC clone that spanned the 
      breakpoint at 12p13 was identified; this YAC included the CDKN1B gene but did not 
      include ETV6. Neither ETV6 nor CDKN1B was rearranged, as determined by FISH and 
      Southern blotting; however, a small deletion encompassing the translocated CDKN1B 
      allele was detected. Analysis of two candidate genes at 10q24, HOX11 and NFKB2 
      suggested that they are not involved in this translocation. The preliminary 
      mapping of breakpoints in this case demonstrated that they are different from an 
      apparently identical translocation identified previously in a patient with 
      myelodysplastic syndrome. The identification of the split YAC and small deletion 
      should enable a more focused search for a gene or genes that may contribute to 
      progression from chronic phase to blast crisis in CML.
FAU - Aguiar, R C
AU  - Aguiar RC
AD  - Department of Haematology, Imperial College School of Medicine, Hammersmith 
      Hospital, London, United Kingdom.
FAU - Chase, A
AU  - Chase A
FAU - Oscier, D G
AU  - Oscier DG
FAU - Carapeti, M
AU  - Carapeti M
FAU - Goldman, J M
AU  - Goldman JM
FAU - Cross, N C
AU  - Cross NC
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p52 Subunit)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 143275-75-6 (TLX1 protein, human)
SB  - IM
MH  - Blotting, Southern
MH  - Chromosome Aberrations/genetics
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - DNA Probes
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Homeodomain Proteins/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - NF-kappa B/genetics
MH  - NF-kappa B p52 Subunit
MH  - Neoplasm Proteins/genetics
MH  - Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-ets
MH  - *Repressor Proteins
MH  - Transcription Factors/genetics
MH  - *Translocation, Genetic
MH  - ETS Translocation Variant 6 Protein
EDAT- 1997/12/31 23:47
MHDA- 2000/06/20 09:00
CRDT- 1997/12/31 23:47
PHST- 1997/12/31 23:47 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/31 23:47 [entrez]
AID - 10.1002/(SICI)1098-2264(199712)20:4<408::AID-GCC13>3.0.CO;2-I [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Dec;20(4):408-11.