PMID- 9409217
OWN - NLM
STAT- MEDLINE
DCOM- 19980122
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 17
IP  - 11
DP  - 1997 Nov
TI  - Basic fibroblast growth factor (bFGF) regulates the expression of the CC 
      chemokine monocyte chemoattractant protein-1 (MCP-1) in autocrine-activated 
      endothelial cells.
PG  - 2471-8
AB  - The CC chemokine monocyte chemoattractant protein (MCP)-1 is induced by 
      inflammatory cytokines and acts as a potent regulator of monocyte trafficking. 
      Monocytes adhere preferentially to migrating endothelial cells in vitro and to 
      endothelial cells at the migration front in vivo after aortic balloon denudation 
      injury. Based on these findings, we analyzed MCP-1 expression in migrating and 
      resting bovine aortic endothelial (BAE) cells and identified prominently 
      upregulated levels of MCP-1 expression in migrating BAE cells. Stimulation of 
      resting BAE cells with 5 ng/mL bFGF resulted in a fourfold induction of MCP-1 
      mRNA expression. The time course of bFGF-induced MCP-1 mRNA expression indicated 
      a rapid and direct stimulation of MCP-1 expression that was detectable 30 minutes 
      after stimulation. Levels of basal MCP-1 expression, as well as upregulated 
      levels of MCP-1 in migrating BAE cells, were downregulated by addition of a 
      neutralizing anti-bFGF monoclonal antibody (1.0 microgram/mL). Digestion of 
      conditioned media of resting BAE cells with collagenase led to a dose-dependent 
      induction of MCP-1 expression in resting BAE cells, which was inhibited > 50% by 
      addition of neutralizing anti-bFGF antibody. Confirmation of the Northern blot 
      experiments by ELISA-based quantitation of MCP-1 protein levels identified 
      threefold to sixfold higher levels of MCP-1 in the supernatants of 
      bFGF-stimulated BAE cells than in unstimulated resting BAE cells. Finally, 
      analysis of MCP-1 expression by in situ hybridization carried out on en face 
      preparations of aortas demonstrated that MCP-1 expression is dramatically 
      upregulated in regenerating endothelial cells in vivo after balloon denudation. 
      Though not establishing a direct causal relation between the preferential 
      adhesion of monocytes to migrating endothelial cells, these findings strongly 
      suggest that autocrine-activated endothelial cell-derived MCP-1 may play a 
      critical role in recruiting monocytes. They furthermore support the concept that 
      bFGF acts as an autocrine regulator of endothelial cell activity and may imply an 
      involvement of bFGF as a mediator of inflammatory cell trafficking.
FAU - Wempe, F
AU  - Wempe F
AD  - Department of Gynecology and Obstetrics, University of Gottingen Medical School, 
      Germany.
FAU - Lindner, V
AU  - Lindner V
FAU - Augustin, H G
AU  - Augustin HG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - EC 3.4.24.- (Collagenases)
SB  - IM
MH  - Angioplasty, Balloon/adverse effects
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Aorta, Thoracic/cytology/injuries
MH  - Cattle
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Collagenases/pharmacology
MH  - Endothelium, Vascular/injuries/*metabolism/pathology
MH  - Fibroblast Growth Factor 1/pharmacology
MH  - Fibroblast Growth Factor 2/antagonists & inhibitors/*pharmacology/physiology
MH  - *Gene Expression Regulation
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Wound Healing
EDAT- 1997/12/31 00:00
MHDA- 1997/12/31 00:01
CRDT- 1997/12/31 00:00
PHST- 1997/12/31 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/12/31 00:00 [entrez]
AID - 10.1161/01.atv.17.11.2471 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1997 Nov;17(11):2471-8. doi: 
      10.1161/01.atv.17.11.2471.