PMID- 9409653
OWN - NLM
STAT- MEDLINE
DCOM- 19980108
LR  - 20190512
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 110
IP  - 3
DP  - 1997 Dec
TI  - Amino-terminal identity of co-existent amyloid and non-amyloid immunoglobulin 
      kappa light chain deposits. A human disease to study alterations of protein 
      conformation.
PG  - 472-8
AB  - Tissue deposition of monoclonal immunoglobulin light chains is a serious 
      complication in some patients with B cell proliferative disorders. The deposits 
      are typically fibrillar and Congophilic in amyloid (AL) and non-fibrillar and 
      Congophobic in light chain deposition disease (LCDD), and rarely coexist in the 
      same patient. From post-mortem tissue of an individual with fibrillar and 
      non-fibrillar kappa light chain deposits in different sites, we separately 
      extracted and analysed biochemically and immunochemically the non-amyloid 
      deposits from isolated glomeruli, the amyloid from isolated renal arteries and 
      the amyloid from myocardium in which the only deposits were amyloid restricted to 
      mural arteries. Western blotting analysis of both the extracted amyloid and the 
      non-amyloid deposits demonstrated 25-kD bands immunoreactive with anti-kappa 
      antibody, and the identity of the N-terminal amino acid sequences that belong to 
      the variable region kappaIV light chain subgroup. This is the first human disease 
      in which antigenically similar but morphologically different deposits have been 
      separately biochemically analysed. We propose that combined LCDD and AL is an 
      ideal human disease to study the relationships and the factors that influence the 
      conversion of non-amyloidogenic to amyloidogenic conformations.
FAU - Kaplan, B
AU  - Kaplan B
AD  - Department of Pathology, New York University Medical Centre, NY 10016, USA.
FAU - Vidal, R
AU  - Vidal R
FAU - Kumar, A
AU  - Kumar A
FAU - Ghiso, J
AU  - Ghiso J
FAU - Frangione, B
AU  - Frangione B
FAU - Gallo, G
AU  - Gallo G
LA  - eng
GR  - AG0805/AG/NIA NIH HHS/United States
GR  - AR 02594/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Amyloid)
RN  - 0 (Immunoglobulin kappa-Chains)
SB  - IM
MH  - Aged
MH  - Amino Acid Sequence
MH  - Amyloid/*chemistry
MH  - Amyloidosis/*metabolism
MH  - Humans
MH  - Hypergammaglobulinemia/*metabolism
MH  - Immunoglobulin kappa-Chains/*chemistry
MH  - Immunohistochemistry
MH  - Male
MH  - Molecular Sequence Data
MH  - Protein Conformation
PMC - PMC1904831
EDAT- 1997/12/31 00:00
MHDA- 1997/12/31 00:01
PMCR- 1998/12/01
CRDT- 1997/12/31 00:00
PHST- 1997/12/31 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/12/31 00:00 [entrez]
PHST- 1998/12/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1997.4421454.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1997 Dec;110(3):472-8. doi: 10.1046/j.1365-2249.1997.4421454.x.