PMID- 9409712
OWN - NLM
STAT- MEDLINE
DCOM- 19980127
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 773
IP  - 1-2
DP  - 1997 Oct 31
TI  - Cholinergic, nitric oxidergic innervation in cerebral arteries of the cat.
PG  - 117-24
AB  - Using immunoperoxidase labeling (IPL) and immunofluorescence labeling (IFL) 
      methods, and each followed by NADPH diaphorase (NADPHd) histochemical staining in 
      the same specimen, colocalization of choline acetyltransferase (ChAT) and NADPHd, 
      indicative of nitric oxide synthase (NOS), in cerebral pial arteries and the 
      sphenopalatine ganglia (SPG) of the cat was examined. In addition, retrograde 
      axonal tracing using true blue was performed to determine if cerebral 
      perivascular nerves containing ChAT and NADPHd originate in the SPG. Consistent 
      results were obtained from IPL and IFL methods, indicating that the middle 
      cerebral artery (MCA) and the circle of Willis received dense ChAT-immunoreactive 
      (I) and NADPHd bundles and fine fibers. Almost all ChAT-I fibers and NADPHd 
      fibers were found to be coincident in the arteries examined. A few fine fibers 
      exhibited only NADPHd staining. In the SPG, approximately half of the ganglionic 
      cells were both ChAT-I and NADPHd positive, while the remaining cells were 
      positively only for NADPHd staining. One week after application of true blue on 
      the middle cerebral arteries (MCA), the fluorescent true blue was found in the 
      ganglionic cells of the SPG. Some of the true blue-positive cells contained both 
      ChAT-immunoreactivity and NADPHd staining. These results provide morphological 
      evidence indicating that all ChAT-I fibers in the MCA and the circle of Willis 
      contain NOS, and that these fibers originate in the SPG, although not all NOS-I 
      ganglionic cells in the SPG send fibers to pial vessels. These results also 
      support the hypothesis that acetylcholine (ACh) and nitric oxide (NO) are 
      synthesized and co-released in the same neurons in cerebral perivascular nerves. 
      Based on the reported findings that NO mediates a major component of neurogenic 
      vasodilation, and that ACh acts as a modulator, the present results demonstrate 
      the presence of a cholinergic, nitric oxidergic innervation in cerebral arteries 
      of the cat.
FAU - Kimura, T
AU  - Kimura T
AD  - Southern Illinois University School of Medicine, Department of Pharmacology, 
      Springfield 62794-1222, USA.
FAU - Yu, J G
AU  - Yu JG
FAU - Edvinsson, L
AU  - Edvinsson L
FAU - Lee, T J
AU  - Lee TJ
LA  - eng
GR  - HL27763/HL/NHLBI NIH HHS/United States
GR  - HL47574/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Cats
MH  - Cerebral Arteries/*innervation
MH  - Choline O-Acetyltransferase/*analysis
MH  - Circle of Willis/innervation
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Ganglia, Parasympathetic/cytology/enzymology
MH  - Immunoenzyme Techniques
MH  - Male
MH  - NADPH Dehydrogenase/*analysis
MH  - Nerve Fibers/enzymology/ultrastructure
MH  - Nitric Oxide Synthase/*analysis
MH  - Pia Mater/blood supply
EDAT- 1997/12/31 00:00
MHDA- 1997/12/31 00:01
CRDT- 1997/12/31 00:00
PHST- 1997/12/31 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/12/31 00:00 [entrez]
AID - S0006-8993(97)00889-5 [pii]
AID - 10.1016/s0006-8993(97)00889-5 [doi]
PST - ppublish
SO  - Brain Res. 1997 Oct 31;773(1-2):117-24. doi: 10.1016/s0006-8993(97)00889-5.