PMID- 9413050
OWN - NLM
STAT- MEDLINE
DCOM- 19980122
LR  - 20190921
IS  - 0941-1291 (Print)
IS  - 0941-1291 (Linking)
VI  - 27
IP  - 11
DP  - 1997
TI  - Analysis of p53 gene deletions in colorectal cancers using fluorescence in situ 
      hybridization.
PG  - 999-1004
AB  - To examine the relationship between the incidence of p53 gene deletion in each 
      nucleus and the clinicopathological features in colorectal cancers, we performed 
      a cytogenetic study using fluorescence in situ hybridization (FISH). FISH was 
      performed on 5 adenomas and 38 colorectal cancers that had been resected 
      surgically. The nucleus, in which the copy number of the p53 signal was lower 
      than that of chromosome 17, was determined as a deletion of the p53 gene. The 
      mean frequency of the deletion of p53 in adenomas and cancers were 7.8% +/- 3.0% 
      and 57.0% +/- 19.0%, respectively. Numerical aberrations of chromosome 17 or a 
      deletion of p53 were also detected in DNA diploidy. The mean frequency of the 
      deletion of p53 in 32 cases with aneusomy of chromosome 17 (65.7% +/- 14.5%) was 
      significantly higher than that in cases of disomy (51.1% +/- 19.3%, P < 0.05). 
      Even though this frequency was high in the early stage, it was not associated 
      with any specific histopathological features. This frequency was also higher in 
      double primary cancers (70.4% +/- 16.7%) compared with single colorectal cancers 
      (53.4% +/- 18.1%) (P < 0.05). Using FISH, our results demonstrated that the 
      clonal deletion of the p53 locus is an early genetic event of colorectal cancers 
      and that a high incidence of p53 deletion may influence the occurrence of double 
      primary cancers.
FAU - Nanashima, A
AU  - Nanashima A
AD  - First Department of Surgery, Nagasaki University School of Medicine, Japan.
FAU - Tagawa, Y
AU  - Tagawa Y
FAU - Yasutake, T
AU  - Yasutake T
FAU - Taniguchi, Y
AU  - Taniguchi Y
FAU - Sawai, T
AU  - Sawai T
FAU - Nakagoe, T
AU  - Nakagoe T
FAU - Ayabe, H
AU  - Ayabe H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Surg Today
JT  - Surgery today
JID - 9204360
SB  - IM
MH  - Adenoma/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Colorectal Neoplasms/*genetics/pathology
MH  - *Gene Deletion
MH  - Gene Dosage
MH  - Gene Frequency
MH  - *Genes, p53
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
EDAT- 1997/01/01 00:00
MHDA- 1997/12/31 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/BF02385778 [doi]
PST - ppublish
SO  - Surg Today. 1997;27(11):999-1004. doi: 10.1007/BF02385778.