PMID- 9413156
OWN - NLM
STAT- MEDLINE
DCOM- 19980120
LR  - 20081121
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 17
IP  - 5A
DP  - 1997 Sep-Oct
TI  - Interleukin 18 enhances Fas ligand expression and induces apoptosis in 
      Fas-expressing human myelomonocytic KG-1 cells.
PG  - 3253-8
AB  - Interleukin-18 (IL-18) induces apoptosis in human myelomonocytic KG-1 cells as 
      determined by agarose gel electrophoresis, and flow cytometry after propidium 
      iodide (PI) staining. Apoptosis was detected 20 hours from the start of culture 
      at concentrations of 100 ng/ml of the cytokine. Although IL-18 induces the 
      production of large amounts of interferon gamma (IFN-gamma) by KG-1 cells, 
      conditioned media could not induce apoptosis of fresh cells. The protein 
      expressions of p53 and Fas ligand by KG-1 cells, which constitutively express the 
      Fas antigen (CD95), were found to increase after exposure to IL-18 for 20 hours. 
      Both Fas ligand and its receptor were found to be functional by in vitro assays 
      on Fas-expressing target cells and an agonist anti-Fas antibody, respectively. In 
      conclusion, IL-18 enhances the expression of Fas ligand by Fas-expressing KG-1 
      cells and induces apoptosis in the cells through a mechanism probably involving 
      the Fas pathway.
FAU - Ohtsuki, T
AU  - Ohtsuki T
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
FAU - Micallef, M J
AU  - Micallef MJ
FAU - Kohno, K
AU  - Kohno K
FAU - Tanimoto, T
AU  - Tanimoto T
FAU - Ikeda, M
AU  - Ikeda M
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Cytokines)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Interleukin-18)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - *Apoptosis
MH  - Cytokines/*physiology
MH  - DNA Fragmentation
MH  - DNA, Neoplasm/metabolism
MH  - Fas Ligand Protein
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-18
MH  - Leukemia, Myelomonocytic, Acute/*pathology
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 1997/12/31 00:00
MHDA- 1997/12/31 00:01
CRDT- 1997/12/31 00:00
PHST- 1997/12/31 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/12/31 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1997 Sep-Oct;17(5A):3253-8.