PMID- 9413615
OWN - NLM
STAT- MEDLINE
DCOM- 19980120
LR  - 20131121
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 11
IP  - 6
DP  - 1997 Nov-Dec
TI  - Stereoselective metabolic study of famprofazone.
PG  - 356-61
AB  - Famprofazone (1) metabolites were studied in human urine after medication by 50 
      mg oral dose. The human urine was collected over 48 h from six volunteers at time 
      intervals of 6, 12, 24 and 48 h. The amount of famprofazone metabolites were 
      recovered from the urine samples by application of Extrelut extraction method. 
      The resultant extracts were derivatized using 
      N-methyl-N-trimethylsilytrifluoroacetamide (MSTFA) for trimethylsilylation 
      followed by N-methyl-bis-trifluoroacetamide (MBTFA) for trifluoroacetylation. 
      Methamphetamine (2) and 3-hydroxymethyl-propyphenazone (3), excreted in human 
      urine, were identified as famprofazone metabolites by gas chromatography-mass 
      spectrometry (GC-MS). The quantitative results revealed that the average amounts 
      of 2 and 3, excreted in human urine were equal to 2.6 and 4 mg, respectively, 
      through 48 h. However, 3 was analysed after enzymatic hydrolysis of the urine 
      samples using beta-glucuronidase/arylsulphatase. The excreted methamphetamine 
      enantiomers could be separated by application of indirect GC-technique using 
      S-(-)-N-trifluoroacetylprolyl chloride (TPC) as a chiral derivatizing agent. The 
      average amount of (-)-methamphetamine isomer excreted in the urine was found to 
      be three fold those of the (+)-isomer.
FAU - Neugebauer, M
AU  - Neugebauer M
AD  - Pharmazeutisches Institut, University of Bonn, Germany.
FAU - Khedr, A
AU  - Khedr A
FAU - el-Rabbat, N
AU  - el-Rabbat N
FAU - el-Kommos, M
AU  - el-Kommos M
FAU - Saleh, G
AU  - Saleh G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyrazolones)
RN  - 44RAL3456C (Methamphetamine)
RN  - HN0NCX453C (famprofazone)
SB  - IM
MH  - Administration, Oral
MH  - Anti-Inflammatory Agents, Non-Steroidal/administration & 
      dosage/pharmacokinetics/*urine
MH  - Biotransformation
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Methamphetamine/administration & dosage/*analogs & 
      derivatives/pharmacokinetics/urine
MH  - Pyrazoles/administration & dosage/pharmacokinetics/*urine
MH  - *Pyrazolones
MH  - Reference Values
MH  - Stereoisomerism
EDAT- 1997/12/31 02:42
MHDA- 2000/06/20 09:00
CRDT- 1997/12/31 02:42
PHST- 1997/12/31 02:42 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/12/31 02:42 [entrez]
AID - 10.1002/(SICI)1099-0801(199711)11:6<356::AID-BMC692>3.0.CO;2-T [pii]
AID - 10.1002/(SICI)1099-0801(199711)11:6<356::AID-BMC692>3.0.CO;2-T [doi]
PST - ppublish
SO  - Biomed Chromatogr. 1997 Nov-Dec;11(6):356-61. doi: 
      10.1002/(SICI)1099-0801(199711)11:6<356::AID-BMC692>3.0.CO;2-T.