PMID- 9413807
OWN - NLM
STAT- MEDLINE
DCOM- 19980120
LR  - 20181113
IS  - 0391-4097 (Print)
IS  - 0391-4097 (Linking)
VI  - 20
IP  - 9
DP  - 1997 Oct
TI  - Sporadic amplification of the insulin receptor gene in human breast cancer.
PG  - 531-6
AB  - Insulin receptor (IR) content is increased in most human breast carcinomas when 
      compared to normal breast tissue. In the present study we investigated IR gene 
      copy number by using both conventional DNA analysis (slot blot) and fluorescence 
      in situ hybridization (FISH). Cultured human breast cell lines and primary breast 
      carcinoma specimens were analyzed. In 6 breast cell lines in culture both 
      techniques gave similar results: the relative IR copy number determined by FISH 
      strongly correlated with slot blot results (r = 0.831), even if probes for 
      different reference loci were used in the 2 methods. We find that in human breast 
      cancer IR gene amplification is a sporadic event. It occurred in 1/5 cultured 
      breast cancer cell lines (MDA-MB 231) and in 8/93 (8.6%) breast cancer specimens. 
      In contrast an increased copy number of the entire chromosome 19 (which contains 
      IR gene) was frequently observed in both breast cancer cell lines (100%) and 
      breast cancer specimens (45%). When present, IR gene amplification always 
      occurred at low level. These data indicate that IR gene amplification is an 
      uncommon event in human breast carcinomas and that mechanisms other than gene 
      amplification are responsible for IR protein overexpression in most human breast 
      cancers.
FAU - Papa, V
AU  - Papa V
AD  - Cattedra di Endocrinologia, Universita di Catania, Ospedale Garibaldi, Italy.
FAU - Milazzo, G
AU  - Milazzo G
FAU - Goldfine, I D
AU  - Goldfine ID
FAU - Waldman, F M
AU  - Waldman FM
FAU - Vigneri, R
AU  - Vigneri R
LA  - eng
GR  - CA44768/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Italy
TA  - J Endocrinol Invest
JT  - Journal of endocrinological investigation
JID - 7806594
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Blotting, Southern
MH  - Breast Neoplasms/*genetics
MH  - Chromosomes, Human, Pair 19
MH  - DNA, Neoplasm/analysis
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization
MH  - Receptor, Insulin/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1997/12/31 00:00
MHDA- 1997/12/31 00:01
CRDT- 1997/12/31 00:00
PHST- 1997/12/31 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/12/31 00:00 [entrez]
AID - 10.1007/BF03348015 [doi]
PST - ppublish
SO  - J Endocrinol Invest. 1997 Oct;20(9):531-6. doi: 10.1007/BF03348015.