PMID- 9416008
OWN - NLM
STAT- MEDLINE
DCOM- 19980219
LR  - 20161026
IS  - 0375-8427 (Print)
IS  - 0375-8427 (Linking)
VI  - 42
IP  - 10
DP  - 1997 Oct
TI  - Isolation and identification of porcine reproductive and respiratory syndrome 
      virus in cell cultures.
PG  - 281-7
AB  - Three strains of porcine reproductive and respiratory syndrome virus (PRRSV) were 
      isolated in porcine lung macrophage (PLM) cultures from three swine herds. This 
      has been the first successful isolation of PRRSV in the Czech Republic and the 
      strains received the designations CAPM V-501, CAPM V-502 and CAPM V-503, 
      respectively. All the three isolates in PLM were identified by immunofluorescence 
      and immunoperoxidase tests and the strain CAPM V-502 also by electron microscopy 
      using the ultrathin section technique. The strain CAPM V-502 has been adapted to 
      the cell line MARC-145. Viral RNA in PLM cultures infected with any of the 
      isolated PRRSV strains was demonstrated by RT-PCR targeted to the more conserved 
      ORF 7 genomic region encoding the nucleocapsid protein. The assessment of PCR 
      products in agarose gel revealed a uniform size of 394 bp in all the three 
      isolates and the European prototype strain Lelystad used as positive control.
FAU - Valicek, L
AU  - Valicek L
AD  - Veterinary Research Institute, Brno, Czech Republic. 
      l.valicek<kahr@vuvel.anet.cz>vuvel/uluva
FAU - Psikal, I
AU  - Psikal I
FAU - Smid, B
AU  - Smid B
FAU - Rodak, L
AU  - Rodak L
FAU - Kubalikova, R
AU  - Kubalikova R
FAU - Kosinova, E
AU  - Kosinova E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Czech Republic
TA  - Vet Med (Praha)
JT  - Veterinarni medicina
JID - 0063417
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cells, Cultured
MH  - Macrophages, Alveolar/virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*isolation & 
      purification
MH  - Swine/*virology
EDAT- 1998/01/07 00:00
MHDA- 1998/01/07 00:01
CRDT- 1998/01/07 00:00
PHST- 1998/01/07 00:00 [pubmed]
PHST- 1998/01/07 00:01 [medline]
PHST- 1998/01/07 00:00 [entrez]
PST - ppublish
SO  - Vet Med (Praha). 1997 Oct;42(10):281-7.