PMID- 9417075
OWN - NLM
STAT- MEDLINE
DCOM- 19980203
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 273
IP  - 1
DP  - 1998 Jan 2
TI  - Hepatocyte growth factor/scatter factor binds with high affinity to dermatan 
      sulfate.
PG  - 271-8
AB  - We have demonstrated by affinity chromatography that hepatocyte growth 
      factor/scatter factor (HGF/SF) binds strongly to dermatan sulfate (DS), with a 
      similar ionic strength dependence to that previously seen with heparan sulfate 
      (HS). Analysis of binding kinetics on a biosensor yields an equilibrium 
      dissociation constant, KD, of 19.7 nM. This corresponds to a 10-100-fold weaker 
      interaction than that with HS, primarily due to a faster dissociation rate of the 
      complex. The smallest DS oligosaccharide with significant affinity for HGF/SF by 
      affinity chromatography appears to be an octasaccharide. A sequence comprising 
      unsulfated iduronate residues in combination with 4-O-sulfated 
      N-acetylgalactosamine is sufficient for high affinity binding. The presence of 
      2-O-sulfation on the iduronate residues does not appear to be inhibitory. These 
      observations concur with our previous suggestions, from analyses of HS binding 
      (Lyon, M., Deakin, J. A., Mizuno, K., Nakamura, T., and Gallagher, J.T. (1994) J. 
      Biol. Chem. 269, 11216-11223), that N-sulfation of hexosamines and 2-O-sulfation 
      of iduronates are not absolute requirements for glycosaminoglycan binding to 
      HGF/SF. This is the first described example of a high affinity interaction 
      between a growth factor and DS, and is likely to have significant implications 
      for the biological activity of this paracrine-acting factor.
FAU - Lyon, M
AU  - Lyon M
AD  - Cancer Research Campaign & University of Manchester, Department of Medical 
      Oncology, Christie Hospital National Health Service Trust, Manchester M20 4BX, 
      United Kingdom. MLyon@picr.man.ac.uk
FAU - Deakin, J A
AU  - Deakin JA
FAU - Rahmoune, H
AU  - Rahmoune H
FAU - Fernig, D G
AU  - Fernig DG
FAU - Nakamura, T
AU  - Nakamura T
FAU - Gallagher, J T
AU  - Gallagher JT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Oligosaccharides)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Binding, Competitive
MH  - Carbohydrate Conformation
MH  - Cell Line
MH  - Dermatan Sulfate/*metabolism
MH  - Dogs
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligosaccharides/chemistry
MH  - Protein Binding
EDAT- 1998/02/07 00:00
MHDA- 1998/02/07 00:01
CRDT- 1998/02/07 00:00
PHST- 1998/02/07 00:00 [pubmed]
PHST- 1998/02/07 00:01 [medline]
PHST- 1998/02/07 00:00 [entrez]
AID - S0021-9258(18)38575-2 [pii]
AID - 10.1074/jbc.273.1.271 [doi]
PST - ppublish
SO  - J Biol Chem. 1998 Jan 2;273(1):271-8. doi: 10.1074/jbc.273.1.271.