PMID- 9418902
OWN - NLM
STAT- MEDLINE
DCOM- 19980122
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 18
IP  - 1
DP  - 1998 Jan
TI  - 4-1BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor 
      receptor subfamily that bind TNF receptor-associated factors and activate nuclear 
      factor kappaB.
PG  - 558-65
AB  - Members of the tumor necrosis factor (TNF)-nerve growth factor (NGF) receptor 
      family have been shown to be important costimulatory molecules for cellular 
      activation. 4-1BB and Ox40 are two recently described members of this protein 
      family which are expressed primarily on activated T cells. To gain insight into 
      the signaling pathways employed by these factors, yeast two-hybrid library 
      screens were performed with the cytoplasmic domains of 4-1BB and Ox40 as baits. 
      TNF receptor-associated factor 2 (TRAF2) was identified as an interacting protein 
      in both screens. The ability of both 4-1BB and Ox40 to interact with TRAF2 was 
      confirmed in mammalian cells by coimmunoprecipitation studies. When the binding 
      of the receptors to other TRAF proteins was investigated, 4-1BB and Ox40 
      displayed distinct binding patterns. While 4-1BB bound TRAF2 and TRAF1, Ox40 
      interacted with TRAF3 and TRAF2. Using deletion and alanine scanning analysis, we 
      defined the elements in the cytoplasmic domains of both receptors that mediate 
      these interactions. The 4-1BB receptor was found to have two independent 
      stretches of acidic residues that can mediate association of the TRAF molecules. 
      In contrast, a single TRAF binding domain was identified in the cytoplasmic tail 
      of Ox40. The cytoplasmic domains of both receptors were shown to activate nuclear 
      factor kappaB in a TRAF-dependent manner. Taken together, our results indicate 
      that 4-1BB and Ox40 bind TRAF proteins to initiate a signaling cascade leading to 
      activation of nuclear factor kappaB.
FAU - Arch, R H
AU  - Arch RH
AD  - Department of Medicine, Howard Hughes Medical Institute, The University of 
      Chicago, Illinois 60637, USA.
FAU - Thompson, C B
AU  - Thompson CB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antigens, CD)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Receptors, OX40)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNFRSF4 protein, human)
RN  - 0 (TNFRSF9 protein, human)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 9)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, CD
MH  - Humans
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Molecular Sequence Data
MH  - NF-kappa B/genetics/*metabolism
MH  - Receptors, Nerve Growth Factor/genetics/*metabolism
MH  - Receptors, OX40
MH  - Receptors, Tumor Necrosis Factor/genetics/*metabolism
MH  - *Signal Transduction
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7/genetics/*metabolism
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 9
PMC - PMC121523
EDAT- 1998/01/07 00:00
MHDA- 1998/01/07 00:01
PMCR- 1998/01/01
CRDT- 1998/01/07 00:00
PHST- 1998/01/07 00:00 [pubmed]
PHST- 1998/01/07 00:01 [medline]
PHST- 1998/01/07 00:00 [entrez]
PHST- 1998/01/01 00:00 [pmc-release]
AID - 0543 [pii]
AID - 10.1128/MCB.18.1.558 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1998 Jan;18(1):558-65. doi: 10.1128/MCB.18.1.558.