PMID- 9419743
OWN - NLM
STAT- MEDLINE
DCOM- 19980202
LR  - 20191102
IS  - 1071-5576 (Print)
IS  - 1071-5576 (Linking)
VI  - 1
IP  - 1
DP  - 1994 Jan-Mar
TI  - Mechanism of cytokine stimulation of prostaglandin biosynthesis in human decidua.
PG  - 31-6
AB  - OBJECTIVE: The purpose of this study was to determine the mechanism of action of 
      the cytokines tumor necrosis factor-alpha (TNF alpha) and interleukin-1 beta 
      (IL-1 beta) on the stimulation of prostaglandin (PG) production in human decidua. 
      METHODS: Decidual cells from term placentas were grown in culture until 
      confluent. Incubations were performed with TNF alpha or IL-1 beta, and with 
      cycloheximide, actinomycin D, arachidonic acid, or acetylsalicylic acid (ASA). 
      Prostaglandin E2 was measured by radioimmunoassay and cellular protein 
      determined. RESULTS: The concentration-related stimulation of decidual PGE2 
      production by IL-1 beta and TNF alpha was completely abrogated by cycloheximide 
      and actinomycin D treatment. Although arachidonic acid alone stimulated decidual 
      PGE2 biosynthesis, the addition of IL-1 beta or TNF alpha consistently augmented 
      this effect. Both cytokines induced recovery of PGE2 biosynthesis from ASA 
      pretreatment more rapidly than controls. CONCLUSIONS: Interleukin-1 beta and TNF 
      alpha both act on decidual PG biosynthesis in a manner requiring new protein 
      synthesis. In combination with our other results, this suggests that IL-1 beta 
      and TNF alpha act to induce PG endoperoxide synthase activity.
FAU - Pollard, J K
AU  - Pollard JK
AD  - Department of Obstetrics and Gynecology, University of Utah School of Medicine, 
      Salt Lake City 84132, USA.
FAU - Thai, D
AU  - Thai D
FAU - Mitchell, M D
AU  - Mitchell MD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Soc Gynecol Investig
JT  - Journal of the Society for Gynecologic Investigation
JID - 9433806
RN  - 0 (Interleukin-1)
RN  - 0 (Prostaglandins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Cycloheximide/pharmacology
MH  - Decidua/*drug effects/metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Pregnancy
MH  - Prostaglandins/*biosynthesis
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Stimulation, Chemical
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1994/01/01 00:00
MHDA- 1998/01/07 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1998/01/07 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1177/107155769400100107 [doi]
PST - ppublish
SO  - J Soc Gynecol Investig. 1994 Jan-Mar;1(1):31-6. doi: 10.1177/107155769400100107.