PMID- 9420287
OWN - NLM
STAT- MEDLINE
DCOM- 19980128
LR  - 20231105
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 72
IP  - 1
DP  - 1998 Jan
TI  - Mechanism of Borna disease virus entry into cells.
PG  - 783-8
AB  - We have investigated the entry pathway of Borna disease virus (BDV). Virus entry 
      was assessed by detecting early viral replication and transcription. 
      Lysosomotropic agents (ammonium chloride, chloroquine, and amantadine), as well 
      as energy depletion, prevented BDV infection, indicating that BDV enters host 
      cells by endocytosis and requires an acidic intracellular compartment to allow 
      membrane fusion and initiate infection. Consistent with this hypothesis, we 
      observed that BDV-infected cells form extensive syncytia upon low-pH treatment. 
      Entry of enveloped viruses into animal cells usually requires the membrane-fusing 
      activity of viral surface glycoproteins (GPs). BDV GP is expressed as two 
      products of 84 and 43 kDa (GP-84 and GP-43, respectively). We show here that only 
      GP-43 is present at the surface of BDV-infected cells and therefore is likely the 
      viral polypeptide responsible for triggering fusion events. We also present 
      evidence that GP-43, which corresponds to the C terminus of GP-84, is generated 
      by cleavage of GP-84 by the cellular protease furin. Hence, we propose that BDV 
      GP-84 is involved in attachment to the cell surface receptor whereas its 
      furin-cleaved product, GP-43, is involved in pH-dependent fusion after 
      internalization of the virion by endocytosis.
FAU - Gonzalez-Dunia, D
AU  - Gonzalez-Dunia D
AD  - Department of Neuropharmacology, The Scripps Research Institute, La Jolla, 
      California 92037, USA.
FAU - Cubitt, B
AU  - Cubitt B
FAU - de la Torre, J C
AU  - de la Torre JC
LA  - eng
GR  - R01 NS032355/NS/NINDS NIH HHS/United States
GR  - NS 32355-02/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Viral Proteins)
RN  - 01Q9PC255D (Ammonium Chloride)
RN  - 886U3H6UFF (Chloroquine)
RN  - BF4C9Z1J53 (Amantadine)
SB  - IM
MH  - Amantadine/pharmacology
MH  - Ammonium Chloride/pharmacology
MH  - Animals
MH  - Borna disease virus/genetics/*pathogenicity/physiology
MH  - Cell Line
MH  - Chloroquine/pharmacology
MH  - Cytopathogenic Effect, Viral
MH  - Endocytosis
MH  - Giant Cells
MH  - Hydrogen-Ion Concentration
MH  - Lysosomes/drug effects
MH  - Membrane Fusion
MH  - Rats
MH  - Transcription, Genetic
MH  - Viral Proteins/physiology
MH  - Virus Replication/drug effects
PMC - PMC109436
EDAT- 1998/01/07 00:00
MHDA- 1998/01/07 00:01
PMCR- 1998/01/01
CRDT- 1998/01/07 00:00
PHST- 1998/01/07 00:00 [pubmed]
PHST- 1998/01/07 00:01 [medline]
PHST- 1998/01/07 00:00 [entrez]
PHST- 1998/01/01 00:00 [pmc-release]
AID - 1352 [pii]
AID - 10.1128/JVI.72.1.783-788.1998 [doi]
PST - ppublish
SO  - J Virol. 1998 Jan;72(1):783-8. doi: 10.1128/JVI.72.1.783-788.1998.