PMID- 9421261
OWN - NLM
STAT- MEDLINE
DCOM- 19980122
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 7
DP  - 1997 Nov
TI  - Karyotype analysis of Helianthus annuus using Giemsa banding and fluorescence in 
      situ hybridization.
PG  - 451-6
AB  - The karyotype of H. annuus was analysed by computer-aided image processing with 
      respect to the chromosome length, arm ratio, occurrence and chromosomal position 
      of intercalary heterochromatin and the location of 18S/25S and 5S ribosomal RNA 
      genes. The karyotype was subdivided into a group of four acrocentric chromosome 
      pairs, of which two were distinguishable by HKG (HCl, KOH, Giemsa) banding and a 
      group of 13 meta- to submetacentric pairs. The latter could be subdivided into 
      seven pairs with one and six pairs with two HKG bands. Three pairs of 
      submetacentric satellite chromosomes revealed 18S/25S rDNA loci after 
      fluorescence in situ hybridization (FISH) and silver staining. A fourth, smaller 
      and possibly inactive, locus occurred in the terminal position on a metacentric 
      pair. One submetacentric satellite chromosome pair revealed a 5S rRNA gene locus 
      in the pericentromeric position; a second locus marked a submetacentric pair with 
      one HKG band. The C-banding technique marks exclusively centromeric 
      heterochromatin. Measurements of chromosomes in combination with Giemsa banding 
      and FISH enabled the discrimination of most chromosome pairs of the sunflower.
FAU - Schrader, O
AU  - Schrader O
AD  - Institut fur Zuchtungsmethodik bei Gemuse der Bundesanstalt fur 
      Zuchtungsforschung an Kulturpflanzen, Quedlinburg, Germany.
FAU - Ahne, R
AU  - Ahne R
FAU - Fuchs, J
AU  - Fuchs J
FAU - Schubert, I
AU  - Schubert I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Azure Stains)
RN  - 0 (Coloring Agents)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Feulgen stain)
RN  - 0 (Rosaniline Dyes)
SB  - IM
MH  - Azure Stains
MH  - *Chromosome Banding
MH  - Chromosomes/chemistry/genetics
MH  - Coloring Agents
MH  - DNA Probes/genetics
MH  - DNA, Ribosomal/genetics
MH  - Helianthus/*genetics
MH  - Image Processing, Computer-Assisted
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Rosaniline Dyes
MH  - Silver Staining
MH  - Staining and Labeling
EDAT- 1998/01/08 00:00
MHDA- 1998/01/08 00:01
CRDT- 1998/01/08 00:00
PHST- 1998/01/08 00:00 [pubmed]
PHST- 1998/01/08 00:01 [medline]
PHST- 1998/01/08 00:00 [entrez]
AID - 10.1023/a:1018412912790 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Nov;5(7):451-6. doi: 10.1023/a:1018412912790.