PMID- 9421265
OWN - NLM
STAT- MEDLINE
DCOM- 19980122
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 5
IP  - 7
DP  - 1997 Nov
TI  - Nature of B chromosomes in the harvest mouse Reithrodontomys megalotis by 
      fluorescence in situ hybridization (FISH).
PG  - 475-9
AB  - Using fluorescence in situ hybridization, we examined the characteristics of two 
      types of B chromosomes in harvest mice of the genus Reithrodontomys. B 
      chromosomes were interrogated with rDNA, telomeric repeat, LINE element and 
      centromeric heterochromatin probes. The two types of B chromosomes share the 
      following features: (a) telomeres present on the ends of both arms; (b) 
      hybridization to LINE probes; (c) absence of hybridization to the ribosomal gene 
      probes; (d) C-band-positive centromeric regions; and (e) euchromatic arms. They 
      differ as follows: (a) the larger B element hybridizes to the centromeric 
      heterochromatin (pMeg-1) probe whereas the smaller B element does not; (b) the 
      amount of C-band-positive material is reduced in the smaller B chromosome 
      relative to that present on the larger B chromosome; and (c) the smaller element 
      is reduced in size by about a third. It is concluded that the larger B chromosome 
      arose as a leftover centromere from centric fusion, whereas the smaller element 
      has a different origin perhaps as an intact fragment or as an amplified region 
      from the A chromosomes. The presence of euchromatic regions on B chromosomes may 
      account for their survival in the karyotype.
FAU - Peppers, J A
AU  - Peppers JA
AD  - Department of Biological Sciences, Texas Tech University, Lubbock 97409-3131, 
      USA. bjjap@ttacs.ttu.edu
FAU - Wiggins, L E
AU  - Wiggins LE
FAU - Baker, R J
AU  - Baker RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Centromere/genetics
MH  - Chromosomes/*genetics
MH  - DNA Probes/genetics
MH  - DNA Transposable Elements/genetics
MH  - DNA, Ribosomal/genetics
MH  - Heterochromatin/genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Sigmodontinae/*genetics
MH  - Telomere
EDAT- 1998/01/08 00:00
MHDA- 1998/01/08 00:01
CRDT- 1998/01/08 00:00
PHST- 1998/01/08 00:00 [pubmed]
PHST- 1998/01/08 00:01 [medline]
PHST- 1998/01/08 00:00 [entrez]
AID - 10.1023/a:1018421114607 [doi]
PST - ppublish
SO  - Chromosome Res. 1997 Nov;5(7):475-9. doi: 10.1023/a:1018421114607.