PMID- 9422330
OWN - NLM
STAT- MEDLINE
DCOM- 19980127
LR  - 20191102
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 130
IP  - 6
DP  - 1997 Dec
TI  - Immunopathologic responses to Aspergillus antigen in interleukin-4 knockout mice.
PG  - 567-75
AB  - Two strains of interleukin-4 (IL-4) gene knockout mice were studied and compared 
      with wild strains to determine the role of IL-4 in the immunopathogenesis of 
      murine allergic aspergillosis. Animals immunized intraperitoneally were 
      subsequently challenged with Aspergillus antigen intranasally. The animals were 
      evaluated for total serum immunoglobulin E (IgE) levels, Aspergillus-specific IgG 
      antibody isotypes, peripheral blood eosinophils, cytokine and chemokine mRNA 
      transcripts in spleen cells, and pulmonary histology. No serum IgE was detected 
      in animals deficient in the IL-4 gene. Aspergillus-specific IgG1 was detected in 
      all animals, while enhanced levels of IgG2a were detected in IL-4 knockout 
      animals challenged with A. fumigatus antigen. There were no differences in the 
      peripheral blood or lung eosinophils in the two groups of mice exposed to A. 
      fumigatus. These results indicate that lung injury in Aspergillus-antigen 
      challenged animals may be the result of the eosinophil mediators and that 
      IgE-mediated injury may not be significant in this model, which may be a 
      significant variation between the model and human allergic aspergillosis.
FAU - Kurup, V P
AU  - Kurup VP
AD  - Department of Medicine, Medical College of Wisconsin, Veterans Affairs Medical 
      Center, Milwaukee 53295, USA.
FAU - Guo, J
AU  - Guo J
FAU - Murali, P S
AU  - Murali PS
FAU - Choi, H
AU  - Choi H
FAU - Fink, J N
AU  - Fink JN
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
CIN - J Lab Clin Med. 1997 Dec;130(6):548-9. PMID: 9422327
MH  - Animals
MH  - Antibodies, Fungal/immunology
MH  - Antigens, Fungal/*immunology
MH  - Aspergillosis, Allergic Bronchopulmonary/*immunology
MH  - Aspergillus fumigatus/*immunology
MH  - Bone Marrow/enzymology
MH  - Chemokines/genetics
MH  - Cytokines/genetics
MH  - Eosinophils/enzymology/*immunology
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - Interleukin-4/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Peroxidase/metabolism
MH  - Spleen/cytology
MH  - Transcription, Genetic
EDAT- 1998/01/09 00:00
MHDA- 1998/01/09 00:01
CRDT- 1998/01/09 00:00
PHST- 1998/01/09 00:00 [pubmed]
PHST- 1998/01/09 00:01 [medline]
PHST- 1998/01/09 00:00 [entrez]
AID - S0022-2143(97)90106-2 [pii]
AID - 10.1016/s0022-2143(97)90106-2 [doi]
PST - ppublish
SO  - J Lab Clin Med. 1997 Dec;130(6):567-75. doi: 10.1016/s0022-2143(97)90106-2.