PMID- 94243
OWN - NLM
STAT- MEDLINE
DCOM- 19800514
LR  - 20190819
IS  - 0361-8609 (Print)
IS  - 0361-8609 (Linking)
VI  - 7
IP  - 2
DP  - 1979
TI  - Subpopulations of human peripheral blood cells: analysis of granulocytic 
      progenitor cells by flow cytometry and immunologic surface markers.
PG  - 163-72
AB  - Normal human peripheral blood cells were separated into different populations 
      based upon isopycnic sedimentation, E rosetting, and EAC rosetting. Each 
      population was characterized according to morphology, surface markers, 
      granulocytic colony formation in semi-solid media, and stainable RNA content by 
      acridine orange (AO) flow cytometry. These techniques enrich for a population of 
      cells that is characterized by a lymphoid morphology, a high 
      granulocytic-macrophage progenitor cell cloning efficiency, a lack of surface 
      markers, and a high stainable RNA content not found in the other two populations 
      of peripheral blood lymphocytes (T cells and B cells). The stainable RNA content 
      serves as a new metabolic marker for the population of cells in which the 
      preponderance of granulocytic progenitor cells reside.
FAU - Drapkin, R L
AU  - Drapkin RL
FAU - Adreeff, M
AU  - Adreeff M
FAU - Koziner, B
AU  - Koziner B
FAU - Strife, A
AU  - Strife A
FAU - Wisniewski, D
AU  - Wisniewski D
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
FAU - Melamed, M R
AU  - Melamed MR
FAU - Clarkson, B
AU  - Clarkson B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Hematol
JT  - American journal of hematology
JID - 7610369
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - B-Lymphocytes/cytology
MH  - Blood Cells/analysis/cytology
MH  - Cell Separation
MH  - Clone Cells/analysis
MH  - Cytological Techniques/*methods
MH  - Fluorescence
MH  - Granulocytes/*cytology
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Lymphocytes/analysis
MH  - RNA/analysis
MH  - Receptors, Antigen, B-Cell/*immunology
MH  - Staining and Labeling
MH  - T-Lymphocytes/cytology
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 10.1002/ajh.2830070209 [doi]
PST - ppublish
SO  - Am J Hematol. 1979;7(2):163-72. doi: 10.1002/ajh.2830070209.