PMID- 9425155
OWN - NLM
STAT- MEDLINE
DCOM- 19980210
LR  - 20200304
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 140
IP  - 1
DP  - 1998 Jan 12
TI  - Detection of dimers of dimers of human leukocyte antigen (HLA)-DR on the surface 
      of living cells by single-particle fluorescence imaging.
PG  - 71-9
AB  - The technique of single-particle fluorescence imaging was used to investigate the 
      oligomeric state of MHC class II molecules on the surface of living cells. Cells 
      transfected with human leukocyte antigen (HLA)-DR A and B genes were labeled at 
      saturation with a univalent probe consisting of Fab coupled to R-phycoerythrin. 
      Analysis of the intensities of fluorescent spots on the cell surface revealed the 
      presence of single and double particles consistent with the simultaneous presence 
      of HLA-DR heterodimers and dimers of dimers. The proportion of double particles 
      was lower at 37 degrees C than at 22 degrees C, suggesting that the heterodimers 
      and dimers of dimers exist in a temperature-dependent equilibrium. These results 
      are discussed in the context of a possible role for HLA-DR dimers of dimers in T 
      cell receptor-MHC interactions. The technique is validated by demonstrating that 
      fluorescence imaging can distinguish between dimers and tetramers of human 
      erythrocyte spectrin deposited from solution onto a solid substrate. The 
      methodology will have broad applicability to investigation of the oligomeric 
      state of immunological and other membrane-bound receptors in living cells.
FAU - Cherry, R J
AU  - Cherry RJ
AD  - Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester 
      CO4 3SQ, United Kingdom. cherr@essex.ac.uk
FAU - Wilson, K M
AU  - Wilson KM
FAU - Triantafilou, K
AU  - Triantafilou K
FAU - O'Toole, P
AU  - O'Toole P
FAU - Morrison, I E
AU  - Morrison IE
FAU - Smith, P R
AU  - Smith PR
FAU - Fernandez, N
AU  - Fernandez N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DR alpha-Chains)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Cell Line
MH  - Cell Membrane/immunology
MH  - Dimerization
MH  - Flow Cytometry
MH  - HLA-DR Antigens/analysis/*biosynthesis/chemistry
MH  - HLA-DR alpha-Chains
MH  - Humans
MH  - Immunoglobulin Fab Fragments
MH  - Leukocytes/immunology
MH  - Macromolecular Substances
MH  - Microscopy, Fluorescence
MH  - Recombinant Proteins/analysis/biosynthesis/chemistry
MH  - Transfection
PMC - PMC2132594
EDAT- 1998/02/14 00:00
MHDA- 1998/02/14 00:01
PMCR- 1998/07/12
CRDT- 1998/02/14 00:00
PHST- 1998/02/14 00:00 [pubmed]
PHST- 1998/02/14 00:01 [medline]
PHST- 1998/02/14 00:00 [entrez]
PHST- 1998/07/12 00:00 [pmc-release]
AID - 10.1083/jcb.140.1.71 [doi]
PST - ppublish
SO  - J Cell Biol. 1998 Jan 12;140(1):71-9. doi: 10.1083/jcb.140.1.71.