PMID- 9425209
OWN - NLM
STAT- MEDLINE
DCOM- 19980218
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 79
IP  - 1
DP  - 1998 Jan
TI  - Facilitation of L-type calcium current in thalamic neurons.
PG  - 410-7
AB  - We have studied facilitation of the L-type calcium current in neurons acutely 
      isolated from the ventrobasal nucleus of the rat thalamus. Currents were recorded 
      after pretreatment with 1 microM omega-conotoxin GVIA and 5 microM 
      omega-conotoxin MVIIC, to better isolate L-current. Long, strong depolarizations 
      induced slow tail currents at negative voltages, but did not affect currents at 
      voltages where channels were strongly activated. The initial peak tail current 
      was not measurably increased. The time course of recovery from facilitation 
      paralleled the time course of the tail current, indicating that facilitation does 
      not outlast channel closing. The kinase inhibitors staurosporine and H-7 and the 
      phosphatase inhibitor okadaic acid had no significant effect on L-current 
      facilitation compared with control, but facilitation was greater with H-7 than 
      with okadaic acid. The guanosine 5'-triphosphate (GTP) analogs GTP-gamma-S and 
      GDP-beta-S did not affect facilitation. We conclude that L-current facilitation 
      in thalamic neurons does not result from Ser/Thr phosphorylation, although 
      phosphorylation may modulate facilitation. This form of facilitation differs 
      kinetically and pharmacologically from facilitation induced by activation of G 
      protein-coupled receptors.
FAU - Kammermeier, P J
AU  - Kammermeier PJ
AD  - Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio 
      44106, USA.
FAU - Jones, S W
AU  - Jones SW
LA  - eng
GR  - NS-24471/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Peptides)
RN  - 0 (Thionucleotides)
RN  - 0 (omega-Conotoxins)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 147794-23-8 (omega-conotoxin-MVIIC)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 71376-97-1 (guanosine 5'-O-(2-thiodiphosphate))
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - 92078-76-7 (omega-Conotoxin GVIA)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology
MH  - Animals
MH  - Calcium Channel Blockers/*pharmacology
MH  - Calcium Channels/drug effects/*physiology
MH  - Calcium Channels, L-Type
MH  - Electrophysiology/methods
MH  - Enzyme Inhibitors/pharmacology
MH  - GTP-Binding Proteins/physiology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Diphosphate/analogs & derivatives/pharmacology
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Membrane Potentials/drug effects
MH  - Neurons/drug effects/*physiology
MH  - Okadaic Acid/pharmacology
MH  - Peptides/*pharmacology
MH  - Rats
MH  - Staurosporine/pharmacology
MH  - Thalamic Nuclei/*physiology
MH  - Thionucleotides/pharmacology
MH  - Time Factors
MH  - omega-Conotoxin GVIA
MH  - *omega-Conotoxins
EDAT- 1998/02/21 00:00
MHDA- 1998/02/21 00:01
CRDT- 1998/02/21 00:00
PHST- 1998/02/21 00:00 [pubmed]
PHST- 1998/02/21 00:01 [medline]
PHST- 1998/02/21 00:00 [entrez]
AID - 10.1152/jn.1998.79.1.410 [doi]
PST - ppublish
SO  - J Neurophysiol. 1998 Jan;79(1):410-7. doi: 10.1152/jn.1998.79.1.410.