PMID- 9426213
OWN - NLM
STAT- MEDLINE
DCOM- 19980126
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 419
IP  - 1
DP  - 1997 Dec 8
TI  - Stabilizing and destabilizing effects of intercalators on DNA triplexes.
PG  - 27-31
AB  - Oligonucleotide-directed triplex formation attracts much attention due to its 
      potential usefulness in diagnostic and biotechnological applications. Among other 
      aspects, the research embraces numerous studies probing the influence of 
      intercalating ligands on triplex stability. The effect of the intercalator on 
      triplex formation and stability is known to depend on nucleotide sequence, type 
      of intercalator and solution conditions. The present work is aimed at determining 
      the average number of intercalated ethidium bromide (EtBr) and acridine orange 
      (AO) molecules leading to the most effective stabilization of triplexes. First, 
      fluorescing complexes of intramolecular parallel (recombinant) triplex 
      5'-d(CATGCTAACT)-L-d(AGTTAGCATG)-L-d(CATGCTAACT)-3' (parARB) and classical 
      antiparallel 5'-(dA)10-L-(dT)10-L-(dT)10-3'(antiATT) (L = -pO(CH2CH2O)3p-) with 
      EtBr and AO were characterized, binding constants were obtained and compared to 
      those for homologous DNA duplexes. Then the total EtBr and AO concentrations 
      corresponding to an average of one, two or three intercalated molecules per 
      oligonucleotide were estimated. Thermal denaturation of parARB and antiATT 
      complexes with an average of one, two or three bound molecules was carried out, 
      thermodynamic parameters of the triplex-to-duplex and duplex-to-open-strand 
      transitions were evaluated using a three-state model. The ability of EtBr and AO 
      to stabilize or destabilize both parallel (recombinant) and classical 
      antiparallel triplexes was found to depend strongly on the concentration of bound 
      intercalator. The triplexes were shown to be stabilized by intercalation of the 
      first and second EtBr or AO molecules, while binding of the third intercalator 
      molecule to 10 nucleotide long triplex resulted in significant triplex 
      destabilization.
FAU - Shchyolkina, A K
AU  - Shchyolkina AK
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow. 
      annas@genome.eimb.rssi.ru
FAU - Borisova, O F
AU  - Borisova OF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Intercalating Agents)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (triplex DNA)
RN  - 9007-49-2 (DNA)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - DNA/*chemistry/*drug effects
MH  - Ethidium/pharmacology
MH  - Fluorescent Dyes
MH  - Hot Temperature
MH  - Intercalating Agents/*pharmacology
MH  - Kinetics
MH  - Nucleic Acid Conformation
MH  - Nucleic Acid Denaturation
MH  - Oligodeoxyribonucleotides
EDAT- 1998/01/13 00:00
MHDA- 1998/01/13 00:01
CRDT- 1998/01/13 00:00
PHST- 1998/01/13 00:00 [pubmed]
PHST- 1998/01/13 00:01 [medline]
PHST- 1998/01/13 00:00 [entrez]
AID - S0014-5793(97)01417-8 [pii]
AID - 10.1016/s0014-5793(97)01417-8 [doi]
PST - ppublish
SO  - FEBS Lett. 1997 Dec 8;419(1):27-31. doi: 10.1016/s0014-5793(97)01417-8.