PMID- 9426513
OWN - NLM
STAT- MEDLINE
DCOM- 19980204
LR  - 20190512
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 109
IP  - 1
DP  - 1998 Jan
TI  - Comparative analysis of interphase FISH and RT-PCR to detect bcr-abl 
      translocation in chronic myelogenous leukemia and related disorders.
PG  - 16-23
AB  - The t(9;22)(q34;q1 1) between the abl and bcr genes plays a pivotal role in the 
      diagnosis and pathogenesis of chronic myelogenous leukemia (CML). Its detection 
      is routinely accomplished by Southern blot analysis and karyotyping. Interphase 
      fluorescence in situ hybridization (FISH) and reverse transcriptase-polymerase 
      chain reaction (RT-PCR) are emerging molecular techniques that offer viable 
      alternatives. We analyzed 40 samples of peripheral blood and bone marrow (CML, 
      16; acute myelogenous leukemia, 6; acute lymphoblastic leukemia [ALL], 1; chronic 
      lymphoblastic leukemia, 2; myelodysplasias, 4; myeloproliferative disorders, 
      unclassified, 3; nonleukemic hematologic malignancies, 3; hypercellular bone 
      marrow, 1; normal control samples, 2; and K562 cell line samples, 2) for the 
      presence of bcr-abl fusion gene and its messenger RNA (mRNA) transcript by FISH 
      and RT-PCR, respectively. We compared the results with results of Southern blot 
      analysis and karyotyping when available. Cost analysis was performed. 
      Thirty-three samples were evaluable by FISH; 14 of 14 evaluable CML samples and 
      one ALL sample were positive for bcr-abl by FISH (100%). The other 15 evaluable 
      samples were negative; 16 of 16 (100%) and 13 of 16 (81%) of CML cases were 
      positive for bcr-abl mRNA by RT-PCR (chemiluminescent blot method) and RT-PCR 
      (colorimetric method), respectively. The ALL sample was positive by both RT-PCR 
      methods. All other samples were negative by RT-PCR (chemiluminescent blot 
      method), and all but 1 case of myeloproliferative disorder tested negative by 
      RT-PCR (colorimetric method). We conclude the utility of FISH and RT-PCR is 
      associated with certain limitations, such as insufficient RNA for RT-PCR and the 
      occasional absence of internal positive FISH control signals. However, each 
      procedure offers (with a high concordance rate) a specific and cost-effective 
      alternative to Southern blot analysis and karyotyping and improved turnaround 
      time for the detection of bcr-abl fusion gene or its mRNA transcript.
FAU - Tbakhi, A
AU  - Tbakhi A
AD  - Department of Clinical Pathology, Cleveland Clinic Foundation, OH 44195, USA.
FAU - Pettay, J
AU  - Pettay J
FAU - Sreenan, J J
AU  - Sreenan JJ
FAU - Abdel-Razeq, H
AU  - Abdel-Razeq H
FAU - Kalaycio, M
AU  - Kalaycio M
FAU - Hoeltge, G
AU  - Hoeltge G
FAU - Miller, M L
AU  - Miller ML
FAU - Tubbs, R R
AU  - Tubbs RR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
RN  - EC 2.7.11.1 (BCR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
SB  - IM
CIN - Am J Clin Pathol. 1998 Jan;109(1):3-5. PMID: 9426510
MH  - Blotting, Southern/economics
MH  - Bone Marrow/chemistry
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - Colorimetry
MH  - Fluorescent Dyes/analysis
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Genes, abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/economics
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Leukocytes/chemistry
MH  - Oncogene Proteins/*genetics
MH  - Polymerase Chain Reaction/economics
MH  - *Protein-Tyrosine Kinases
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-bcr
MH  - RNA, Messenger/analysis
MH  - RNA, Neoplasm/analysis
MH  - Transcription, Genetic
MH  - *Translocation, Genetic
EDAT- 1998/01/14 00:00
MHDA- 1998/01/14 00:01
CRDT- 1998/01/14 00:00
PHST- 1998/01/14 00:00 [pubmed]
PHST- 1998/01/14 00:01 [medline]
PHST- 1998/01/14 00:00 [entrez]
AID - 10.1093/ajcp/109.1.16 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1998 Jan;109(1):16-23. doi: 10.1093/ajcp/109.1.16.