PMID- 9426599 OWN - NLM STAT- MEDLINE DCOM- 19980126 LR - 20190822 IS - 0167-4412 (Print) IS - 0167-4412 (Linking) VI - 35 IP - 6 DP - 1997 Dec TI - Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza. PG - 791-9 AB - Large variation in genome size as determined by the nuclear DNA content and the mitotic chromosome size among diploid rice species is revealed using flow cytometry and image analyses. Both the total chromosomal length (r = 0.939) and the total chromosomal area (r = 0.927) correlated well with the nuclear DNA content. Among all the species examined, Oryza australiensis (E genome) and O. brachyantha (F genome), respectively, were the largest and smallest in genome size. O. sativa (A genome) involving all the cultivated species showed the intermediate genome size between them. The distribution patterns of genome-specific repetitive DNA sequences were physically determined using fluorescence in situ hybridization (FISH). O. brachyantha had limited sites of the repetitive DNA sequences specific to the F genome. O. australiensis showed overall amplification of genome-specific DNA sequences throughout the chromosomes. The amplification of the repetitive DNA sequences causes the variation in the chromosome morphology and thus the genome size among diploid species in the genus Oryza. FAU - Uozu, S AU - Uozu S AD - Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station, Joetsu, Japan. FAU - Ikehashi, H AU - Ikehashi H FAU - Ohmido, N AU - Ohmido N FAU - Ohtsubo, H AU - Ohtsubo H FAU - Ohtsubo, E AU - Ohtsubo E FAU - Fukui, K AU - Fukui K LA - eng PT - Journal Article PL - Netherlands TA - Plant Mol Biol JT - Plant molecular biology JID - 9106343 RN - 0 (DNA, Plant) SB - IM MH - Base Sequence MH - Chromosomes/chemistry/*genetics MH - DNA, Plant/analysis MH - Flow Cytometry MH - *Genetic Variation MH - *Genome, Plant MH - Image Processing, Computer-Assisted MH - In Situ Hybridization, Fluorescence MH - Microscopy, Fluorescence MH - Mitosis/genetics MH - Molecular Sequence Data MH - Oryza/cytology/*genetics MH - *Repetitive Sequences, Nucleic Acid EDAT- 1998/01/14 00:00 MHDA- 1998/01/14 00:01 CRDT- 1998/01/14 00:00 PHST- 1998/01/14 00:00 [pubmed] PHST- 1998/01/14 00:01 [medline] PHST- 1998/01/14 00:00 [entrez] AID - 10.1023/a:1005823124989 [doi] PST - ppublish SO - Plant Mol Biol. 1997 Dec;35(6):791-9. doi: 10.1023/a:1005823124989.