PMID- 9427999
OWN - NLM
STAT- MEDLINE
DCOM- 19980217
LR  - 20190914
IS  - 0141-9838 (Print)
IS  - 0141-9838 (Linking)
VI  - 19
IP  - 11
DP  - 1997 Nov
TI  - Production of IgE antibodies against the 22 kDa tegumental membrane-associated 
      antigen of schistosomes is directed by the antigen itself.
PG  - 531-3
AB  - It has previously been reported that the predominant target of immunoglobulin E 
      (IgE) recognition in sera from humans infected with Schistosoma japonicum in The 
      Philippines or with S. mansoni in Kenya, is a 22 kDa tegumental 
      membrane-associated schistosome antigen. In the present study, we demonstrate 
      that the 22 kDa antigen can direct the production of antigen-specific IgE 
      antibodies independently of schistosome infection and in the absence of any other 
      parasite components or adjuvant. Three strains of mice were immunized using the 
      purified, recombinant 22 kDa antigen of S. japonicum without the use of any 
      adjuvant. Sera from all three strains of immunized mice, but not control animals, 
      generated IgE antibodies specific for the native 22 kDa schistosome antigen in 
      Western blots. Thus, the 22 kDa antigen itself must contain signals (presumably 
      encoded by the primary amino acid sequence or by the secondary or tertiary 
      structures of the molecule, or by a combination of these) which are sufficient to 
      direct the isotype switch required for production of antigen-specific IgE.
FAU - Waine, G J
AU  - Waine GJ
AD  - Molecular Parasitology Unit, Queensland Institute of Medical Research, Post 
      Office Royal Brisbane Hospital, Australia.
FAU - Mazzer, D R
AU  - Mazzer DR
FAU - McManus, D P
AU  - McManus DP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/biosynthesis/*immunology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases
MH  - Immunoglobulin E/biosynthesis/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Recombinant Fusion Proteins/immunology
MH  - Schistosoma japonicum/*immunology
MH  - Schistosoma mansoni/*immunology
EDAT- 1998/01/15 00:00
MHDA- 1998/01/15 00:01
CRDT- 1998/01/15 00:00
PHST- 1998/01/15 00:00 [pubmed]
PHST- 1998/01/15 00:01 [medline]
PHST- 1998/01/15 00:00 [entrez]
AID - 10.1046/j.1365-3024.1997.d01-164.x [doi]
PST - ppublish
SO  - Parasite Immunol. 1997 Nov;19(11):531-3. doi: 10.1046/j.1365-3024.1997.d01-164.x.