PMID- 9428356
OWN - NLM
STAT- MEDLINE
DCOM- 19980202
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 100
IP  - 2
DP  - 1998 Jan 15
TI  - Sequential flow cytometry and fluorescence in situ hybridization for the study of 
      formalin-fixed, paraffin-embedded breast cancer cells.
PG  - 129-33
AB  - Both flow cytometry and fluorescence in situ hybridization (FISH) are useful 
      techniques in the analysis of cancer tissues. When the two are used in the study 
      of the same specimens, they are usually performed in parallel, separately. This 
      is problematic where there is a scarcity of material, making completion of both 
      studies impossible. Fluorescence in situ hybridization procedures that will 
      utilize excess material discarded from flow cytometry would be advantageous. The 
      present report describes an optimized protocol for performing sequential flow 
      cytometry and FISH using formalin-fixed paraffin-embedded archival material. 
      Although breast cancer tissues were used in this initial study, the protocol is 
      applicable to other cancer tissues as well.
FAU - Mark, H F
AU  - Mark HF
AD  - Department of Pathology, Rhode Island Hospital, Providence 02903, USA.
FAU - Rausch, M E
AU  - Rausch ME
FAU - Taylor, W M
AU  - Taylor WM
FAU - Huth, A
AU  - Huth A
FAU - Mark, S
AU  - Mark S
FAU - Santoro, K
AU  - Santoro K
FAU - Zolnierz, K
AU  - Zolnierz K
FAU - Ferreira, K
AU  - Ferreira K
FAU - Barker, B E
AU  - Barker BE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Flow Cytometry/*methods
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Paraffin Embedding
EDAT- 1998/01/15 00:00
MHDA- 1998/01/15 00:01
CRDT- 1998/01/15 00:00
PHST- 1998/01/15 00:00 [pubmed]
PHST- 1998/01/15 00:01 [medline]
PHST- 1998/01/15 00:00 [entrez]
AID - S0165-4608(97)00018-6 [pii]
AID - 10.1016/s0165-4608(97)00018-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Jan 15;100(2):129-33. doi: 
      10.1016/s0165-4608(97)00018-6.